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| Description
| - This study was aimed at comparison of some sperm characteristics in Czech Fleckvieh (n= 8) and Holstein (n= 8) bulls at the age of 1 to 6 years. After determination of pathologic sperm (staining with Congo red, Bromothymol blue and Janus green) and sperm motility, the samples of semen with progressive movement >=70% were diluted in AndroMed extender containing soybean lecithin extract, frozen in a programmable freezing device and stored in liquid nitrogen until analyses. Sperm motility was analyzed by CASA. Sperm plasma membrane integrity and apoptosis incidence were detected under a Leica fluorescent microscope after staining with PNA-Alexa Fluor and Yo-Pro-I in combination with propidium iodide (PI), using specific wavelength filters. Average age of bulls and incidence of pathological sperm were not different between CF and H breeds, whilst average body condition score (BCS) was significantly higher (p< 0,05) in CF (2.84±0, 17) than in H (2.19±0, 13) bulls. Total sperm motility and progressive movement measured immediately after thawing (38.6±1.8 and 34.19±1.9, resp) and following 0,5 h (46.44±1.79 and 44,14±1.8) were higher in H bulls compared to CF bulls (34,15±2 and 30.27±2,13; 39,17 ±2 and 36.12±2.07, resp.), but following 2 h these traits were not different. More sperm with plasma membrane damages were recorded in CF bulls (36.41±1.81%) compared to H bulls (26.33± 1.63%). Similarly, higher incidence of apoptotic sperm was noted in CF bulls (19.12±1.71%) compared to H bulls (12.58± 1.51 %). We observed differences in sperm motility and viability between two tested breeds, which may be a result of interbreed difference in average body condition. Makarevič., A. – Špaleková, E. – Stádník, L. – Kubovičová, E. – Louda, F.: Breed differences in several characteristics of bull frozen-thawed sperm. 10th Int. Sci. Conference Animal Physiology 2013 (June 4th-6th 2013; Crocus-Kežmarské Žľaby). In: J. Microbiol. Biotechnol. Food Sci., 2013:2 (Special Issue), s. 6.
- This study was aimed at comparison of some sperm characteristics in Czech Fleckvieh (n= 8) and Holstein (n= 8) bulls at the age of 1 to 6 years. After determination of pathologic sperm (staining with Congo red, Bromothymol blue and Janus green) and sperm motility, the samples of semen with progressive movement >=70% were diluted in AndroMed extender containing soybean lecithin extract, frozen in a programmable freezing device and stored in liquid nitrogen until analyses. Sperm motility was analyzed by CASA. Sperm plasma membrane integrity and apoptosis incidence were detected under a Leica fluorescent microscope after staining with PNA-Alexa Fluor and Yo-Pro-I in combination with propidium iodide (PI), using specific wavelength filters. Average age of bulls and incidence of pathological sperm were not different between CF and H breeds, whilst average body condition score (BCS) was significantly higher (p< 0,05) in CF (2.84±0, 17) than in H (2.19±0, 13) bulls. Total sperm motility and progressive movement measured immediately after thawing (38.6±1.8 and 34.19±1.9, resp) and following 0,5 h (46.44±1.79 and 44,14±1.8) were higher in H bulls compared to CF bulls (34,15±2 and 30.27±2,13; 39,17 ±2 and 36.12±2.07, resp.), but following 2 h these traits were not different. More sperm with plasma membrane damages were recorded in CF bulls (36.41±1.81%) compared to H bulls (26.33± 1.63%). Similarly, higher incidence of apoptotic sperm was noted in CF bulls (19.12±1.71%) compared to H bulls (12.58± 1.51 %). We observed differences in sperm motility and viability between two tested breeds, which may be a result of interbreed difference in average body condition. Makarevič., A. – Špaleková, E. – Stádník, L. – Kubovičová, E. – Louda, F.: Breed differences in several characteristics of bull frozen-thawed sperm. 10th Int. Sci. Conference Animal Physiology 2013 (June 4th-6th 2013; Crocus-Kežmarské Žľaby). In: J. Microbiol. Biotechnol. Food Sci., 2013:2 (Special Issue), s. 6. (en)
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| Title
| - Breed differences in several characteristics of bull frozen-thawed sperm
- Breed differences in several characteristics of bull frozen-thawed sperm (en)
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| skos:prefLabel
| - Breed differences in several characteristics of bull frozen-thawed sperm
- Breed differences in several characteristics of bull frozen-thawed sperm (en)
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| skos:notation
| - RIV/49608851:_____/13:#0000593!RIV14-MZE-49608851
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/49608851:_____/13:#0000593
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Holstein; Czech Fleckvieh; bull; sperm; motility; plasma membrane; apoptosis (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Louda, František
- Stádník, Luděk
- Kubovičová, Elena
- Makarevič, Alexander
- Špaleková, Eliška
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