About: Effect of donor cell and trichostatin A (TSA) on development of cloned dairy cattle embryos

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About: Effect of donor cell and trichostatin A (TSA) on development of cloned dairy cattle embryos Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The objective was to investigate the effects of treatments to donor cells with fresh digestion (FD), cryopreservation/thawning (CT), trichostatin A (TSA) and durations of culture using TSACR1aa medium on in vitro development of dairy cow cloned embryos. The results showed that pretreatment of donor cells using TSA could significantly increase both cleavage and blastocyst rates of embyos (P < 0.05) compared with FD and CT group while no significant difference was found between FD and CT group. When cloned embryos were subjected to TSA treatment in CR1aa for different times (0, 24 , 48 and 60 h), the results showed that the blastocyst rate in the 60 h group was the highest (36.11±1.78%) compared with the other groups (P < 0.05). The objective was to investigate the effects of treatments to donor cells with fresh digestion (FD), cryopreservation/thawning (CT), trichostatin A (TSA) and durations of culture using TSACR1aa medium on in vitro development of dairy cow cloned embryos. The results showed that pretreatment of donor cells using TSA could significantly increase both cleavage and blastocyst rates of embyos (P < 0.05) compared with FD and CT group while no significant difference was found between FD and CT group. When cloned embryos were subjected to TSA treatment in CR1aa for different times (0, 24 , 48 and 60 h), the results showed that the blastocyst rate in the 60 h group was the highest (36.11±1.78%) compared with the other groups (P < 0.05). (en)
Title	Effect of donor cell and trichostatin A (TSA) on development of cloned dairy cattle embryos Effect of donor cell and trichostatin A (TSA) on development of cloned dairy cattle embryos (en)
skos:prefLabel	Effect of donor cell and trichostatin A (TSA) on development of cloned dairy cattle embryos Effect of donor cell and trichostatin A (TSA) on development of cloned dairy cattle embryos (en)
skos:notation	RIV/49608851:_____/12:#0000549!RIV13-MSM-49608851
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ME09081)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Kučera, Josef Hanuš, Oto
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav pro chov skotu, s.r.o.
http://linked.open...dnocenehoVysledku	133008
http://linked.open...ai/riv/idVysledku	RIV/49608851:_____/12:#0000549
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	trichostatin A; somatic cell nuclear transfer; dairy cow (en)
http://linked.open.../riv/klicoveSlovo	dairy cow trichostatin A somatic cell nuclear transfer
http://linked.open...odStatuVydavatele	CN - Čínská lidová republika
http://linked.open...ontrolniKodProRIV	[6102305CD56A]
http://linked.open...i/riv/nazevZdroje	Journal of Agricultural Biotechnology
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Research and technological support of keeping and breeding of cattle with dual yield, milk and beef, in Anhui province
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Hanuš, Oto Kučera, Josef Tao, Yong Hongguo, Cao Meiling, Zhang Xiaorong, Zhang Ya, Liu Yunhai, Zhang Yunsheng, Liu
issn	1674-7968
number of pages	9 (xsd:int)

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