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| rdf:type
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| Description
| - Aims: The livestock milk and meat industry represent a possible source of MRSA strains, with potential to infect the humans or infiltrate the food chain. The aim of the study was to apply different molecular genetic methods to type acquired MRSA strains Methods and results: Hundreds of samples of cow, goat and sheep milk, pork meat and farm personnel were collected in two years in the Czech Republic. Eight MRSA strains from different farms were purchased and analysed using MLST (Multi Locus Sequence typing), spa and SCCmec (Staphylococcal Chromosome Cassette mec) typing based on PCR. The (GTG)5 primer was applied for the rep-PCR analyse. The ribotypization of the strains was performed on the RiboPrinterTM system. The individual strains possessed resistance to 3-8 different antibiotics, two isolates carried the gene seb encoding enterotoxin B. The strains belonged to the 4, 4D and 5 SCCmec type; t011, t034 and t064 spa type and ST8, ST398 MLST type. The results of ribotypization revealed a presence of six ribogroupes in tested strains. The obtained fingerprints were strain specific, the cluster analysis revealed 90-98% similarity among the strains. The rep-PCR indicated presence of five types of strains. Conclusions: The ribotyping of MRSA presented sensitive method with possibility to select individual strains. The rep-PCR was powerful tool for strains selection as well. However, the reproducibility was partly affected with the composition of PCR. The tested MRSA strains belonged to different types of MLST, spa and SCCmec profiles. Significance of the study: Our results pointed out for existence of considerable heterogeneous group of MRSA strains in environment of milk and meat industry in the Czech Republic. This indicates different origin of MRSA strains and presumption of their various infiltration.
- Aims: The livestock milk and meat industry represent a possible source of MRSA strains, with potential to infect the humans or infiltrate the food chain. The aim of the study was to apply different molecular genetic methods to type acquired MRSA strains Methods and results: Hundreds of samples of cow, goat and sheep milk, pork meat and farm personnel were collected in two years in the Czech Republic. Eight MRSA strains from different farms were purchased and analysed using MLST (Multi Locus Sequence typing), spa and SCCmec (Staphylococcal Chromosome Cassette mec) typing based on PCR. The (GTG)5 primer was applied for the rep-PCR analyse. The ribotypization of the strains was performed on the RiboPrinterTM system. The individual strains possessed resistance to 3-8 different antibiotics, two isolates carried the gene seb encoding enterotoxin B. The strains belonged to the 4, 4D and 5 SCCmec type; t011, t034 and t064 spa type and ST8, ST398 MLST type. The results of ribotypization revealed a presence of six ribogroupes in tested strains. The obtained fingerprints were strain specific, the cluster analysis revealed 90-98% similarity among the strains. The rep-PCR indicated presence of five types of strains. Conclusions: The ribotyping of MRSA presented sensitive method with possibility to select individual strains. The rep-PCR was powerful tool for strains selection as well. However, the reproducibility was partly affected with the composition of PCR. The tested MRSA strains belonged to different types of MLST, spa and SCCmec profiles. Significance of the study: Our results pointed out for existence of considerable heterogeneous group of MRSA strains in environment of milk and meat industry in the Czech Republic. This indicates different origin of MRSA strains and presumption of their various infiltration. (en)
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| Title
| - Molecular characterization of MRSA strains isolated from livestock milk and meat in the Czech Republic
- Molecular characterization of MRSA strains isolated from livestock milk and meat in the Czech Republic (en)
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| skos:prefLabel
| - Molecular characterization of MRSA strains isolated from livestock milk and meat in the Czech Republic
- Molecular characterization of MRSA strains isolated from livestock milk and meat in the Czech Republic (en)
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| skos:notation
| - RIV/49608851:_____/11:#0000511!RIV12-MZE-49608851
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/49608851:_____/11:#0000511
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - MRSA, livestock, Czech Republic, molecular characterization (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Manga, Ivan
- Vyletělová, Marcela
- Karpíšková, R.
- Šťástková, Z.
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