About: Estimation of the putrescine and cadaverine production in E.coli strains using the PCR methods

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About: Estimation of the putrescine and cadaverine production in E.coli strains using the PCR methods Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In our study, the ornithine (speC) and lysine (cadA) decarboxylase genes were investigated in E. coli strains using the PCR methods. 26 E. coli strains isolated from raw cow, sheep and goat bulk milk samples and cheeses were evaluated. The alignment of the multiple sequences and analysis of conserved domains in the studied genes was carried out using ClustalW and BLAST programs. The conserved ODC (ornithine decarboxylase like family) domain was selected as a target for primer design of the both genes. Analysis of the sequence sources for the speC gene revealed existence of speC isosyme, featured with homology between the 66 - 98 % comparising to the speC nucleotide alignment. Also, in the case of cadA gene, we observed two groups of sequences with the homology of approximately 60% (GenBank, cadA1, cadA2). On the basement of these facts, we designed and performed also additional PCR tests. 81 % strains were positive for speC, 75 % for speC isozyme gene, 54 % for cadA1 and 45 % for cadA2 PCR test. In our study, the ornithine (speC) and lysine (cadA) decarboxylase genes were investigated in E. coli strains using the PCR methods. 26 E. coli strains isolated from raw cow, sheep and goat bulk milk samples and cheeses were evaluated. The alignment of the multiple sequences and analysis of conserved domains in the studied genes was carried out using ClustalW and BLAST programs. The conserved ODC (ornithine decarboxylase like family) domain was selected as a target for primer design of the both genes. Analysis of the sequence sources for the speC gene revealed existence of speC isosyme, featured with homology between the 66 - 98 % comparising to the speC nucleotide alignment. Also, in the case of cadA gene, we observed two groups of sequences with the homology of approximately 60% (GenBank, cadA1, cadA2). On the basement of these facts, we designed and performed also additional PCR tests. 81 % strains were positive for speC, 75 % for speC isozyme gene, 54 % for cadA1 and 45 % for cadA2 PCR test. (en)
Title	Estimation of the putrescine and cadaverine production in E.coli strains using the PCR methods Estimation of the putrescine and cadaverine production in E.coli strains using the PCR methods (en)
skos:prefLabel	Estimation of the putrescine and cadaverine production in E.coli strains using the PCR methods Estimation of the putrescine and cadaverine production in E.coli strains using the PCR methods (en)
skos:notation	RIV/49608851:_____/10:#0000405!RIV11-MSM-49608851
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(2B08069), P(LA10029)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Manga, Ivan Vyletělová, Marcela
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav pro chov skotu, s.r.o.
http://linked.open...dnocenehoVysledku	257576
http://linked.open...ai/riv/idVysledku	RIV/49608851:_____/10:#0000405
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	putrescine; cadaverine; E.coli; PCR (en)
http://linked.open.../riv/klicoveSlovo	putrescine PCR cadaverine E.coli
http://linked.open...ontrolniKodProRIV	[14F96A74A473]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Relationship research of contaminant microflora and biogenic amines as the risk toxicants in the system of cheese health unexceptionability on the consumer market Support of the membership and cooperation with the Society for Applied Microbiology (SfAM).
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Manga, Ivan Vyletělová, Marcela

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