About: A Role of Hsp70 in Response of Rabbit Preimplantation Embryos to Hyperthermia.

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About: A Role of Hsp70 in Response of Rabbit Preimplantation Embryos to Hyperthermia. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cílem této studie bylo určit roli Hsp70 v odpovědi králičích preimplantačních embryí (Es) k hyperthermii (HT) in vitro. Vystavení Es teplotě 41.5 oC před nebo po diferenciaci blastocyst (DB) neovlivnilo podíl blastocyst (Bl), T-index a počet embryonálních buněk. Blokování Hsp70 před DB ukázalo, že Es (0%) se nevyvíjela do stádia Bl. Z počtu Es Hsp70-blokovaných po DB, se 27.6% (ST+anti-Hsp) a 22.6% (ET+anti-Hsp) formovalo do Bl, oproti Es Hsp70 neblokovaným to bylo 71.4% (ST) a 69.7% (ET). (cs) This study determined a role of Hsp70 in response of rabbit preimplantation embryos (Es) to hyperthermia (HT) in vitro. The exposure of Es to 41.5 oC either before or after DB did not affect blastocysts (Bl) rate, T-index and E cell number. Blocking of Hsp70 before DB resulted in no Es (0%) at Bl stage. From Hsp70-blocked Es after DB, 27.6% (ST+anti-Hsp) and 22.6% (ET+anti-Hsp) of Bl were formed, versus 71.4% (ST) and 69.7% (ET) in non-Hsp70-blocked Es. T-index of Hsp70-blocked Es before DB increased from 6.8% (ST) to 28.6% (ST+anti-Hsp) and from 6.1% (ET) to 29.6% (ET+anti-Hsp), whilst cell number was dropped from 111 +- 8.1 (ST) to 20.5 +- 2.42 (ST+anti-Hsp70) and from 106.8 +- 8.4 (ET) to 15.4 +- 1.34 (ET+anti-Hsp70). Oppositely, in Hsp70-blocked Es after DB, neither TUNEL-index nor cell number were changed comparing to non-blocked Es. This study determined a role of Hsp70 in response of rabbit preimplantation embryos (Es) to hyperthermia (HT) in vitro. The exposure of Es to 41.5 oC either before or after DB did not affect blastocysts (Bl) rate, T-index and E cell number. Blocking of Hsp70 before DB resulted in no Es (0%) at Bl stage. From Hsp70-blocked Es after DB, 27.6% (ST+anti-Hsp) and 22.6% (ET+anti-Hsp) of Bl were formed, versus 71.4% (ST) and 69.7% (ET) in non-Hsp70-blocked Es. T-index of Hsp70-blocked Es before DB increased from 6.8% (ST) to 28.6% (ST+anti-Hsp) and from 6.1% (ET) to 29.6% (ET+anti-Hsp), whilst cell number was dropped from 111 +- 8.1 (ST) to 20.5 +- 2.42 (ST+anti-Hsp70) and from 106.8 +- 8.4 (ET) to 15.4 +- 1.34 (ET+anti-Hsp70). Oppositely, in Hsp70-blocked Es after DB, neither TUNEL-index nor cell number were changed comparing to non-blocked Es. (en)
Title	Role Hsp70 v odezvě králičích preimplantačních embryíi k hypertermii. (cs) A Role of Hsp70 in Response of Rabbit Preimplantation Embryos to Hyperthermia. A Role of Hsp70 in Response of Rabbit Preimplantation Embryos to Hyperthermia. (en)
skos:prefLabel	Role Hsp70 v odezvě králičích preimplantačních embryíi k hypertermii. (cs) A Role of Hsp70 in Response of Rabbit Preimplantation Embryos to Hyperthermia. A Role of Hsp70 in Response of Rabbit Preimplantation Embryos to Hyperthermia. (en)
skos:notation	RIV/49608851:_____/07:8P009144!RIV08-MZE-49608851
http://linked.open.../vavai/riv/strany	107
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(1B44034)
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Kubovičová, Elena Makarevič, Alexander Pivko, Juraj
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav pro chov skotu, s.r.o.
http://linked.open...dnocenehoVysledku	408161
http://linked.open...ai/riv/idVysledku	RIV/49608851:_____/07:8P009144
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Hyperthermia; blastocyst; development (en)
http://linked.open.../riv/klicoveSlovo	Hyperthermia development blastocyst
http://linked.open...ontrolniKodProRIV	[7B06039A065F]
http://linked.open...v/mistoKonaniAkce	Celle,Germany
http://linked.open...i/riv/mistoVydani	neuvedeno
http://linked.open...i/riv/nazevZdroje	Reproduction in domestic animals
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	The effective cryopreservation of in vitro bovine embryos applied in cattle reproduction and breeding improvement.
http://linked.open...UplatneniVysledku	2007
http://linked.open...iv/tvurceVysledku	Kubovičová, Elena Pivko, Juraj Chrenek, P. Olexiková, L. Makarevich, Alexandr
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2007-01-01 (xsd:date)
issn	0936-6768
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Blackwell Verlag

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