About: ŠVÁBOVÁ, L., GRIGA, M., HORÁČEK, J., ONDŘEJ, V., ŠERHANTOVÁ, V.: Methodological aspect of pea (Pisum sativum L.) genetic transformation.

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About: ŠVÁBOVÁ, L., GRIGA, M., HORÁČEK, J., ONDŘEJ, V., ŠERHANTOVÁ, V.: Methodological aspect of pea (Pisum sativum L.) genetic transformation. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Three basic regeneration protocols were used in a set of experiments focused on the development of reliable, routine and genotype-independent transformation method in pea:(1) multiple shoot culture (MSC) derived from axillary meristems, (2) somatic embryogenesis (SE) from apical meristems,(3) trimmed germinating seeds (TGS). The indirect transformation via Agrobacterium tumefaciens (EHA 105) carrying plasmids pBIN19 and pGT89 was used for the basic studies. SE-based Agrobacterium transformation approach resulted in the lowest transformation efficiency (TE)1-2%, TE of MSC and TGS was 18 % and 30 % in T0 generation, respectively. SE regeneration protocol was also used for the experiments with direct gene transfer via particle bombardement with plasmid pDB1. Variants of pressure / grid combinations were studied for optimal particle gun setting. The efficiency of transformation via particle bombardement was 0,5-1,4 %. Three basic regeneration protocols were used in a set of experiments focused on the development of reliable, routine and genotype-independent transformation method in pea:(1) multiple shoot culture (MSC) derived from axillary meristems, (2) somatic embryogenesis (SE) from apical meristems,(3) trimmed germinating seeds (TGS). The indirect transformation via Agrobacterium tumefaciens (EHA 105) carrying plasmids pBIN19 and pGT89 was used for the basic studies. SE-based Agrobacterium transformation approach resulted in the lowest transformation efficiency (TE)1-2%, TE of MSC and TGS was 18 % and 30 % in T0 generation, respectively. SE regeneration protocol was also used for the experiments with direct gene transfer via particle bombardement with plasmid pDB1. Variants of pressure / grid combinations were studied for optimal particle gun setting. The efficiency of transformation via particle bombardement was 0,5-1,4 %. (en)
Title	ŠVÁBOVÁ, L., GRIGA, M., HORÁČEK, J., ONDŘEJ, V., ŠERHANTOVÁ, V.: Methodological aspect of pea (Pisum sativum L.) genetic transformation. ŠVÁBOVÁ, L., GRIGA, M., HORÁČEK, J., ONDŘEJ, V., ŠERHANTOVÁ, V.: Methodological aspect of pea (Pisum sativum L.) genetic transformation. (en)
skos:prefLabel	ŠVÁBOVÁ, L., GRIGA, M., HORÁČEK, J., ONDŘEJ, V., ŠERHANTOVÁ, V.: Methodological aspect of pea (Pisum sativum L.) genetic transformation. ŠVÁBOVÁ, L., GRIGA, M., HORÁČEK, J., ONDŘEJ, V., ŠERHANTOVÁ, V.: Methodological aspect of pea (Pisum sativum L.) genetic transformation. (en)
skos:notation	RIV/48392952:_____/03:00007139!RIV/2004/MSM/AGRSS4/N
http://linked.open.../vavai/riv/strany	89
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ME 433)
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Ondřej, Vladan Horáček, Jiří Griga, Miroslav Švábová, Lenka Šerhantová, Vendula
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	AGRITEC, výzkum, šlechtění a služby, s.r.o.
http://linked.open...dnocenehoVysledku	629854
http://linked.open...ai/riv/idVysledku	RIV/48392952:_____/03:00007139
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	pea;genetic transformation;agrobacterium;biolistic;cocultivation additives (en)
http://linked.open.../riv/klicoveSlovo	pea biolistic genetic transformation agrobacterium cocultivation additives
http://linked.open...ontrolniKodProRIV	[D93A96B8A270]
http://linked.open...v/mistoKonaniAkce	Stará Lesná, Slovakia
http://linked.open...i/riv/mistoVydani	Nitra, Slovakia
http://linked.open...i/riv/nazevZdroje	Plant Biotechnology: Progress and Developments, Book of Abstracts
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20433
http://linked.open...UplatneniVysledku	2003
http://linked.open...iv/tvurceVysledku	Griga, Miroslav Horáček, Jiří Ondřej, Vladan Švábová, Lenka Šerhantová, Vendula
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2003-09-07 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Ústav genetiky a biotechnológií rastlín SAV
https://schema.org/isbn	80-89088-15-5

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