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| rdf:type
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| Description
| - Vybraný soubor 20 meziliniových F1 hybridů Brassica napus L. bylo hodnoceno z hlediska jejich schopnosti produkovat dihaploidní (DH) rostliny odvozené z mikrospórové kultury následně využitých ve šlechtitelském projektu Česká Řepka. Všechny testované genotypy byly schopny regenerovat embrya z mikrospór podle standardizovaného protokolu, který byl již dříve upraven pro odrůdy/šlechtitelské linie České Řepky; byly zjištěny významné rozdíly v tvorbě embryí mezi jednotlivými genotypy. Fertilní DH regeneranti byly získáni z 12 linií souboru. Ostaních 8 linií v podstatě netvořilo DH rostliny z různých důvodů, zejména v důsledku změněné morfologie mikrosporórových embryí ve spojení s omezenou schopností klíčení/konverze a v důsledku neúspěšného ošetření kolchicinem, které mělo za následek haploidní nebo mixoploidní (chimerické) regeneranty vykazující částečnou nebo úplnou sterilitu. Obecně lze konstatovat, že kolchicinové ošetření idukovalo diploidní, polyploidní, (triploidní, tetraploidní) a mixoploi (cs)
- The selected set of twenty inter-line F1 hybrids of Brassica napus L. was evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in the breeding „Czech Winter Rape''. All tested genotypes were able to produce microspore-derived embryos based on the standardized protocol previously adopted for the Czech winter rape varieties/breeding lines; nevertheless, significant differences were found in the frequency of microspore-derived embryos production as related to particular genotypes. However, the fertile dihaploid DH regenerants were obtained only in 12 hybrid lines of this set. The other 8 lines failed to produce fertile DH plants for several reasons, mainly due to altered morphology of microspore embryos connected with limited germination/conversion potential and due to unsuccessful colchicine treatment resulting in haploid or mixoploid (chimeric) regenerants exhibiting partial or full sterility. In general, colchicine treatment induced diploid
- The selected set of twenty inter-line F1 hybrids of Brassica napus L. was evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in the breeding „Czech Winter Rape''. All tested genotypes were able to produce microspore-derived embryos based on the standardized protocol previously adopted for the Czech winter rape varieties/breeding lines; nevertheless, significant differences were found in the frequency of microspore-derived embryos production as related to particular genotypes. However, the fertile dihaploid DH regenerants were obtained only in 12 hybrid lines of this set. The other 8 lines failed to produce fertile DH plants for several reasons, mainly due to altered morphology of microspore embryos connected with limited germination/conversion potential and due to unsuccessful colchicine treatment resulting in haploid or mixoploid (chimeric) regenerants exhibiting partial or full sterility. In general, colchicine treatment induced diploid (en)
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| Title
| - Účinnost mikrosporové kultury na tvorbu dihaploidů ve šlechtitelském projektu ''Česká řepka”. Czech J. Genet. Plant Breed., 42, 58-71, ISSN 1212-1975. (cs)
- Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project ''Czech Winter Rape”. Czech J. Genet. Plant Breed., 42, 58-71, ISSN 1212-1975.
- Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project ''Czech Winter Rape”. Czech J. Genet. Plant Breed., 42, 58-71, ISSN 1212-1975. (en)
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| skos:prefLabel
| - Účinnost mikrosporové kultury na tvorbu dihaploidů ve šlechtitelském projektu ''Česká řepka”. Czech J. Genet. Plant Breed., 42, 58-71, ISSN 1212-1975. (cs)
- Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project ''Czech Winter Rape”. Czech J. Genet. Plant Breed., 42, 58-71, ISSN 1212-1975.
- Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project ''Czech Winter Rape”. Czech J. Genet. Plant Breed., 42, 58-71, ISSN 1212-1975. (en)
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| skos:notation
| - RIV/47116099:_____/06:R0090916!RIV07-MZE-47116099
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/47116099:_____/06:R0090916
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - microspore culture, doubled haploids, winter rape, Brassica napus L., hybrid breeding (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Czech Journal of Genetics and Plant Breeding
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Griga, Miroslav
- Macháčková, Ivana
- Smýkalová, Iva
- Větrovcová, Martina
- Klíma, Miroslav
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| issn
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| number of pages
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