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| Description
| - Induction of haploid zygotic embryogenesis and androgenesis was studied in 72 and 31 wheat genotypes, respectively. Green zygotic plants (haploids only) were obtained in 52 genotypes (72 %), and green androgenic regenerants (haploids and spontaneous polyhaploids) were observed in 17 genotypes (55 %). In zygotic embryogenesis embryos developed in 65 genotypes (90%), and in androgenesis embryos or microsporial calli were detected in 28 genotypes (90 %). The highest frequency of haploid zygotic regenerants was 0.24 per pollinated flower, whilst that of androgenic regenerants was 0.08 per cultured anther (0.24 per three anthers in one flower). On average, in induced androgenesis the plants were produced by one out of three androgenic anthers. In induced haploid zygotic embryogenesis the plants were regenerated by every third flower (ovary) with induced embryos. In a set of selected genotypes the yield of induced haploid zygotic embryogenesis was compared with induced pollen embryogenesis. The productivity
- Induction of haploid zygotic embryogenesis and androgenesis was studied in 72 and 31 wheat genotypes, respectively. Green zygotic plants (haploids only) were obtained in 52 genotypes (72 %), and green androgenic regenerants (haploids and spontaneous polyhaploids) were observed in 17 genotypes (55 %). In zygotic embryogenesis embryos developed in 65 genotypes (90%), and in androgenesis embryos or microsporial calli were detected in 28 genotypes (90 %). The highest frequency of haploid zygotic regenerants was 0.24 per pollinated flower, whilst that of androgenic regenerants was 0.08 per cultured anther (0.24 per three anthers in one flower). On average, in induced androgenesis the plants were produced by one out of three androgenic anthers. In induced haploid zygotic embryogenesis the plants were regenerated by every third flower (ovary) with induced embryos. In a set of selected genotypes the yield of induced haploid zygotic embryogenesis was compared with induced pollen embryogenesis. The productivity (en)
- U českých šlechtitelských materiálů pšenice (Triticum aestivum L.) jsme testovali možnost indukovat haploidii a spontánní polyhaploidii jak sprášením emaskulovaných květů pšenice pylem kukuřice (haploidní zygotickou embryogenezí in vitro) u 72 genotypů, tak pomocí prašníkové kultury (androgenezí in vitro) u 31 genotypů. Raná pšeničná embrya extirpovaná z vyvíjejících se ovarií byla kultivována in vitro a dopěstována in situ, androgenní regeneranti byli odvozováni standardní kultivací prašníků in vitro a dopěstováni in situ. Ploidie všech regenerantů byla stanovována průtokovou cytometrií. Celistvé rostliny se podařilo odvodit haploidní zygotickou embryogenezí u 52 z testovaných genotypů (72 %), androgenezí in vitro u 17 z testovaných genotypů (55 %). Haploidní zygotická embrya se vytvořila u 65 z testovaných genotypů (90 %), pylová embrya nebo mikrosporiální kalusy u 28 testovaných genotypů (90 %). Největší frekvence tvorby celistvých haploidních zygotických regenerantů byla 0,24 rostlin na jeden emas (cs)
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| Title
| - In vitro haploid zygotic embryogenesis due to pollination with maize pollen and induced in vitro androgenesis in Czech wheat breeding genotypes
- In vitro haploid zygotic embryogenesis due to pollination with maize pollen and induced in vitro androgenesis in Czech wheat breeding genotypes (en)
- Haploidní zygotická embryogeneze indukovaná pylem kukuřice a androgeneze in vitro u českých šlechtitelských genotypů pšenice (cs)
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| skos:prefLabel
| - In vitro haploid zygotic embryogenesis due to pollination with maize pollen and induced in vitro androgenesis in Czech wheat breeding genotypes
- In vitro haploid zygotic embryogenesis due to pollination with maize pollen and induced in vitro androgenesis in Czech wheat breeding genotypes (en)
- Haploidní zygotická embryogeneze indukovaná pylem kukuřice a androgeneze in vitro u českých šlechtitelských genotypů pšenice (cs)
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| skos:notation
| - RIV/46904841:_____/01:00010293!RIV/2005/GA0/048415/N
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/46904841:_____/01:00010293
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - wheat genotypes;induced embryogenesis;maize pollen;haploid zygote (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Plant, Soil and Environment
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
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| issn
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| number of pages
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