About: Metabolic pathways of Pseudomonas fluorescens ICT in the perspective of groundwater remediation

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Insufficiency of information on the factors controlling the growth and metabolic capabilities of groundwater bacteria still limits successful implementation of enhanced bioremediation technologies. Genome of P. fluorescens ICT was analysed by 454-pyrosequencing technology. In total, 121 Mb were obtained by two sequencing runs on GS Junior System. The assembly of 454-pyrosequencing data resulted in a construction of 73 contigs with an average length of 99,068 bps. MG-RAST and Blast2Go were used for gene annotations. The predicted size of the genome was 7.2 MB. Of the total genome sequence, 97.2% were proteins annotated by MG-RAST, 91.2% of annotated features were assigned to functional categories. Tested laboratory strain was found to be closely related to the P. fluorescens Pf-5 (Accession No. CP000076) genome with 96% of genes with E below 10-30. Among others, the bacterium contains 18 genes involved in biphenyl metabolism, 10 involved in the metabolism of chlorinated aromatic compounds and 9 genes for naphthalene and anthracene metabolism. Insufficiency of information on the factors controlling the growth and metabolic capabilities of groundwater bacteria still limits successful implementation of enhanced bioremediation technologies. Genome of P. fluorescens ICT was analysed by 454-pyrosequencing technology. In total, 121 Mb were obtained by two sequencing runs on GS Junior System. The assembly of 454-pyrosequencing data resulted in a construction of 73 contigs with an average length of 99,068 bps. MG-RAST and Blast2Go were used for gene annotations. The predicted size of the genome was 7.2 MB. Of the total genome sequence, 97.2% were proteins annotated by MG-RAST, 91.2% of annotated features were assigned to functional categories. Tested laboratory strain was found to be closely related to the P. fluorescens Pf-5 (Accession No. CP000076) genome with 96% of genes with E below 10-30. Among others, the bacterium contains 18 genes involved in biphenyl metabolism, 10 involved in the metabolism of chlorinated aromatic compounds and 9 genes for naphthalene and anthracene metabolism. (en)
Title	Metabolic pathways of Pseudomonas fluorescens ICT in the perspective of groundwater remediation Metabolic pathways of Pseudomonas fluorescens ICT in the perspective of groundwater remediation (en)
skos:prefLabel	Metabolic pathways of Pseudomonas fluorescens ICT in the perspective of groundwater remediation Metabolic pathways of Pseudomonas fluorescens ICT in the perspective of groundwater remediation (en)
skos:notation	RIV/46747885:24620/13:#0000379!RIV14-MPO-24620___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(FR-TI4/278)
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Ševců, Alena Sakmaryová, Iva
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Technická univerzita v Liberci / Ústav pro nanomateriály, pokročilé technologie a inovace
http://linked.open...dnocenehoVysledku	87459
http://linked.open...ai/riv/idVysledku	RIV/46747885:24620/13:#0000379
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Pseudomonas fluorescens, bioremediation, metabolic pathways, genome sequencing (en)
http://linked.open.../riv/klicoveSlovo	bioremediation metabolic pathways Pseudomonas fluorescens genome sequencing
http://linked.open...ontrolniKodProRIV	[DF20DC3BEE1E]
http://linked.open...in/vavai/riv/obor	DK
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	*The R&D and verification of a technology applicable to remediate comprihensively contaminated soils
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Ševců, Alena Sakmaryová, Iva Baldrian, P. Chroňáková, A.
http://localhost/t...ganizacniJednotka	24620

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