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Description
| - Medical and biological applications such as tissue engineering have high demands on materials. Nanofibers made from polyvinyl alcohol and poly (ε-caprolactone) are biocompatible, biodegradable and have specific structural properties. Tissue engineering requires specific chemical properties, which can be obtained by low-pressure microwave plasma surface functionalization. Before such functionalization it is necessary to sterilize the substrate. The aim of this paper is a study of nanofiber functionalization and sterilization of their surface to create functional reactive chemical complex for tissue engineering. These two steps should be performed in one technological process. Experimental conditions were found in order to avoid degradation of nanofibers. Degradation was examined by SEM. For sterilization study, nanofibers were vaccinated with Escherichia Coli and Staphylococcus Aureus bacteria. Sterilization efficiency was evaluated according to the number of bacterial colonies restored by incubation. Electron Spectroscopy for Chemical Analysis (ESCA) was used for detailed determination of the incorporated functional groups. Sterilization of nanofiber surface using optimized conditions was fully successful after 30 or 60 seconds treatment. Incorporation of hydroxyl and carboxyl groups increased by up to 9 percent. The applicability of low-pressure microwave plasma treatment for the surface sterilization and functionalisation was demonstrated.
- Medical and biological applications such as tissue engineering have high demands on materials. Nanofibers made from polyvinyl alcohol and poly (ε-caprolactone) are biocompatible, biodegradable and have specific structural properties. Tissue engineering requires specific chemical properties, which can be obtained by low-pressure microwave plasma surface functionalization. Before such functionalization it is necessary to sterilize the substrate. The aim of this paper is a study of nanofiber functionalization and sterilization of their surface to create functional reactive chemical complex for tissue engineering. These two steps should be performed in one technological process. Experimental conditions were found in order to avoid degradation of nanofibers. Degradation was examined by SEM. For sterilization study, nanofibers were vaccinated with Escherichia Coli and Staphylococcus Aureus bacteria. Sterilization efficiency was evaluated according to the number of bacterial colonies restored by incubation. Electron Spectroscopy for Chemical Analysis (ESCA) was used for detailed determination of the incorporated functional groups. Sterilization of nanofiber surface using optimized conditions was fully successful after 30 or 60 seconds treatment. Incorporation of hydroxyl and carboxyl groups increased by up to 9 percent. The applicability of low-pressure microwave plasma treatment for the surface sterilization and functionalisation was demonstrated. (en)
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Title
| - Investigation of Plasma Treatment of Nanofiber Fabric
- Investigation of Plasma Treatment of Nanofiber Fabric (en)
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skos:prefLabel
| - Investigation of Plasma Treatment of Nanofiber Fabric
- Investigation of Plasma Treatment of Nanofiber Fabric (en)
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skos:notation
| - RIV/46747885:24620/13:#0000355!RIV14-MSM-24620___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/46747885:24620/13:#0000355
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Nanofibers, plasma, biocompatible, biodegradable, ESCA (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
| - Mezinárodní konference Society of Vacuum Coaters
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http://linked.open...i/riv/mistoVydani
| - Albuquerque, NM 87122-1914 USA
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http://linked.open...i/riv/nazevZdroje
| - 2013 Society of Vacuum Coaters 505/856-7188, 56th Annual Technical Conference Proceedings
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Horáková, Marta
- Špatenka, Petr
- Zajícová, Veronika
- Černý, Pavel
- Cerman, Jiří
- Lovětinská - Šlamborová, Irena
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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issn
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number of pages
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http://purl.org/ne...btex#hasPublisher
| - Society of Vacuum Coaters
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http://localhost/t...ganizacniJednotka
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