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| Description
| - This study was undertaken to clarify the morphology, histochemistry, and lectin affinity of the seminiferous epithelial cells and Leydig cells in pre-pubertal and adult domestic turkeys. Antibodies to c-kit were localized to spermatogonia in the pre-pubertal and to a lesser extent in adult males. Peanut agglutinin (PNA) was specific for spermatocytes in the pre-pubertal males and spermatogonia and early spermatocytes in adult males. Wheat-germ agglutinin (WGA) highlighted Sertoli cells in both age groups. Concanavalin A staining was diffuse and not cell-specific and could not be used to selectively identify a particular cell type. It was concluded that WGA and PNA could aid in identifying specific cell types in the seminiferous epithelium of testis from pre-pubertal and mature turkeys. The information from this study is being used to adapt techniques for the isolation and partial purification developed for mammalian spermatogonia to avian spermatogonia and other specific cell types in the testes.
- This study was undertaken to clarify the morphology, histochemistry, and lectin affinity of the seminiferous epithelial cells and Leydig cells in pre-pubertal and adult domestic turkeys. Antibodies to c-kit were localized to spermatogonia in the pre-pubertal and to a lesser extent in adult males. Peanut agglutinin (PNA) was specific for spermatocytes in the pre-pubertal males and spermatogonia and early spermatocytes in adult males. Wheat-germ agglutinin (WGA) highlighted Sertoli cells in both age groups. Concanavalin A staining was diffuse and not cell-specific and could not be used to selectively identify a particular cell type. It was concluded that WGA and PNA could aid in identifying specific cell types in the seminiferous epithelium of testis from pre-pubertal and mature turkeys. The information from this study is being used to adapt techniques for the isolation and partial purification developed for mammalian spermatogonia to avian spermatogonia and other specific cell types in the testes. (en)
- Cílem práce bylo osvětlit morfologii, hstochemii a lektinovou afinitusemenotvorných epitelií a Leydigových buněk krocana domácího. Protilátky proti c-kit byly lokalizovány na spermatogoniích pre-pubertálních i dospělých jedinců. Agglutinin PNA byl specifický pro spermatocyty u pre-pubertálních a spermatogonie a rané spermatocyty dospělců. Agglutinin WGA značil Sertoliho buňky u obou skupin. Značení konkavalinem A bylo difúzní a nespecifické a nemohlo být použito k určení jednotlivých buněčných typů. Z práce vyplývá, že PNA a WGA mohou přispět k identifikaci specifických typů buněk semnotvorných epitelií varlat krocanů. (cs)
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| Title
| - Morphological and histochemical characterization of the seminiferous epithelial and Leydig cells of the turkey
- Morphological and histochemical characterization of the seminiferous epithelial and Leydig cells of the turkey (en)
- Morfologická a histochemická charakterizace semenotvorných epiteliálních a Leydigových buněk krocana (cs)
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| skos:prefLabel
| - Morphological and histochemical characterization of the seminiferous epithelial and Leydig cells of the turkey
- Morphological and histochemical characterization of the seminiferous epithelial and Leydig cells of the turkey (en)
- Morfologická a histochemická charakterizace semenotvorných epiteliálních a Leydigových buněk krocana (cs)
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| skos:notation
| - RIV/46356606:_____/07:#0000016!RIV09-MSM-46356606
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/46356606:_____/07:#0000016
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - turkey; spermatogonial stem cells; seminiferous epithelium (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Animal Reproduction Science
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Trefil, Pavel
- Bakst, M. R.
- Brillard, J.-P.
- Akuffo, V.
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| issn
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| number of pages
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