About: High-performance size-exclusion chromatography of humic substances on the hydroxyethyl methacrylate column     Goto   Sponge   NotDistinct   Permalink

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Description
  • HEMA, the copolymer of 2-hydroxyethyl methacrylate and ethylene dimethacrylate, has proven to be a suitable stationary phase for the size-exclusion chromatographic (SEC) characterization of humic substances in a wide pH range. The SEC measurements were performed typically with alkaline borate-based mobile phase: 0.025 mol/l Na2B4O7, 0.025 mol/l NaCl, 0.001 mol/l Na4P2O7, pH 9.1. After calibrating with globular protein standards, apparent molecular weights of typical peat- and coal-derived humic acids were estimated. The average molecular weights determined with the aid of the HEMA column were quite comparable with the values obtained previously by the method of low-pressure gel-permeation chromatography on the Sephadex column.
  • HEMA, the copolymer of 2-hydroxyethyl methacrylate and ethylene dimethacrylate, has proven to be a suitable stationary phase for the size-exclusion chromatographic (SEC) characterization of humic substances in a wide pH range. The SEC measurements were performed typically with alkaline borate-based mobile phase: 0.025 mol/l Na2B4O7, 0.025 mol/l NaCl, 0.001 mol/l Na4P2O7, pH 9.1. After calibrating with globular protein standards, apparent molecular weights of typical peat- and coal-derived humic acids were estimated. The average molecular weights determined with the aid of the HEMA column were quite comparable with the values obtained previously by the method of low-pressure gel-permeation chromatography on the Sephadex column. (en)
  • Kopolymer HEMA byl použit jako stacionární fáze při vysokoúčinné vylučovací chromatografii (SEC) huminových látek. Jako mobilní fáze při SEC frakcionaci byla použita mobilní fáze na bázi boritanového pufru. Chromatografický systém je použitelný pro charakterizaci huminových látek v širokém rozmezí pH. Po kalibraci pomocí proteinových standardů byla SEC použita k měření zdánlivých molekulových hmotností typických huminových látek připravených z hnědého uhlí a rašeliny. Výsledky byly v dobré shodě s výsledky získanými metodou nízkotlaké permeační gelové chromatografie na koloně Sephadex. (cs)
Title
  • High-performance size-exclusion chromatography of humic substances on the hydroxyethyl methacrylate column
  • Vysokoúčinná vylučovací chromatografie huminových látek na hydroxyethylmethakrylátové koloně (cs)
  • High-performance size-exclusion chromatography of humic substances on the hydroxyethyl methacrylate column (en)
skos:prefLabel
  • High-performance size-exclusion chromatography of humic substances on the hydroxyethyl methacrylate column
  • Vysokoúčinná vylučovací chromatografie huminových látek na hydroxyethylmethakrylátové koloně (cs)
  • High-performance size-exclusion chromatography of humic substances on the hydroxyethyl methacrylate column (en)
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  • RIV/44555601:13520/07:00003746!RIV08-MSM-13520___
http://linked.open.../vavai/riv/strany
  • 160-165
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  • P(1M0554)
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  • 1-2
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  • 424148
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  • RIV/44555601:13520/07:00003746
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  • size-exclusion chromatography; humic substances; HEMA copolymer; molecular weights (en)
http://linked.open.../riv/klicoveSlovo
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  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [5EB31C01BFF2]
http://linked.open...i/riv/nazevZdroje
  • Journal Of Chromatography A
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  • 1160
http://linked.open...iv/tvurceVysledku
  • Janoš, Pavel
  • Zatřepálková, Ivana
issn
  • 0021-9673
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  • 13520
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