About: Oriented and reversible immobilisation of natural and recombinant photosystem II on gold microarray - potentials for use as biosensors for detection of herbicides

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About: Oriented and reversible immobilisation of natural and recombinant photosystem II on gold microarray - potentials for use as biosensors for detection of herbicides Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Příspěvek popisuje současný stav vývoje elektrochemického biočipu pro detekci herbicidů. (cs) Electrochemical on-chip deposition of proteins for further biodetection of several analytes by multiplexed chronoamperometry in a continuous flow m-chamber is presented. Recently, an original deposition procedure was developed obtaining oriented and reversible immobilisation of an engineered photosystem II with an histidine-tag and its on-chip purification from bacterial crude extracts. The electrochemically assisted method started with the deposition of cysteamin on gold or platinum surfaces and further chemical synthesis of a spacer with a Ni-NTA end capable to link the engineered proteins. This technique is applied on a m-chip assembled in a m-chamber with an histidine-tagged alkaline phosphatase. Electrochemical deposition allowed this protein to be addressed only on a specific electrode in the m-array in few minutes and under flow condition (flow-rate=10 µl/min). Electrochemical on-chip deposition of proteins for further biodetection of several analytes by multiplexed chronoamperometry in a continuous flow m-chamber is presented. Recently, an original deposition procedure was developed obtaining oriented and reversible immobilisation of an engineered photosystem II with an histidine-tag and its on-chip purification from bacterial crude extracts. The electrochemically assisted method started with the deposition of cysteamin on gold or platinum surfaces and further chemical synthesis of a spacer with a Ni-NTA end capable to link the engineered proteins. This technique is applied on a m-chip assembled in a m-chamber with an histidine-tagged alkaline phosphatase. Electrochemical deposition allowed this protein to be addressed only on a specific electrode in the m-array in few minutes and under flow condition (flow-rate=10 µl/min). (en)
Title	Oriented and reversible immobilisation of natural and recombinant photosystem II on gold microarray - potentials for use as biosensors for detection of herbicides Oriented and reversible immobilisation of natural and recombinant photosystem II on gold microarray - potentials for use as biosensors for detection of herbicides (en) Orientovaná a vratná imobilizace přirozeného a rekombinantního fotosystému II na povrchu micročipu (cs)
skos:prefLabel	Oriented and reversible immobilisation of natural and recombinant photosystem II on gold microarray - potentials for use as biosensors for detection of herbicides Oriented and reversible immobilisation of natural and recombinant photosystem II on gold microarray - potentials for use as biosensors for detection of herbicides (en) Orientovaná a vratná imobilizace přirozeného a rekombinantního fotosystému II na povrchu micročipu (cs)
skos:notation	RIV/44555601:13430/04:00002624!RIV/2005/GA0/134305/N
http://linked.open.../vavai/riv/strany	88-91
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA522/03/0659)
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Malý, Jan
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Jana Evangelisty Purkyně v Ústí nad Labem / Pedagogická fakulta
http://linked.open...dnocenehoVysledku	578441
http://linked.open...ai/riv/idVysledku	RIV/44555601:13430/04:00002624
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	biochips;microarrays;photosystem II (en)
http://linked.open.../riv/klicoveSlovo	biochips microarrays photosystem II
http://linked.open...ontrolniKodProRIV	[B38ADF2099F2]
http://linked.open...v/mistoKonaniAkce	Ferrara, Italy
http://linked.open...i/riv/mistoVydani	Amsterdam
http://linked.open...i/riv/nazevZdroje	The 9th national conference on sensors and microsystems AISEM
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Improvement of photosynthetic biosenzors and bioassays for routine herbicide residues detection and non-target organisms risk assessment
http://linked.open...UplatneniVysledku	2004
http://linked.open...iv/tvurceVysledku	Malý, Jan Pilloton, R. Masci, A. Vastarella, W. Ilie, M. Foglietti, V. Sigiura, M.
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2004-02-08 (xsd:date)
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Elsevier Science
http://localhost/t...ganizacniJednotka	13430

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