About: Enhanced accumulation of Cadmium in Linum ussitatissimum L. plants due to overproduction of metallothionein α-domain as a fusion to β-glucuronidase gene protein

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Enhanced accumulation of Cadmium in Linum ussitatissimum L. plants due to overproduction of metallothionein α-domain as a fusion to β-glucuronidase gene protein Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/article/10.1007/s11240-012-0239-1
Description	In an attempt to enhance the phytoextraction capacity of L. usitatissimum through overproduction of an efficient heterologous Cd-binding peptide, we engineered linseed breeding line AGT 917 to constitutively express genetic fusion of α-domain of mammalian metallothionein 1a (αMT1a) and β-glucuronidase gus gene under the control of CaMV 35S promoter. An improved transformation protocol was developed. When tested in soils amended with Cd at 20 and 360 mg kg-1, the mature αMT1a::gus plants accumulated more Cd than parental AGT 917: the stem Cd concentrations in the best performing αMT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of αMT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15 mg Cd l-1, indicating that αMT1a::gus did confer higher Cd tolerance to engineered plant. Overproduction of metal-binding peptides thus appears to be a viable strategy for the production of L. usitatissimum with improved phytoremediation capacity In an attempt to enhance the phytoextraction capacity of L. usitatissimum through overproduction of an efficient heterologous Cd-binding peptide, we engineered linseed breeding line AGT 917 to constitutively express genetic fusion of α-domain of mammalian metallothionein 1a (αMT1a) and β-glucuronidase gus gene under the control of CaMV 35S promoter. An improved transformation protocol was developed. When tested in soils amended with Cd at 20 and 360 mg kg-1, the mature αMT1a::gus plants accumulated more Cd than parental AGT 917: the stem Cd concentrations in the best performing αMT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of αMT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15 mg Cd l-1, indicating that αMT1a::gus did confer higher Cd tolerance to engineered plant. Overproduction of metal-binding peptides thus appears to be a viable strategy for the production of L. usitatissimum with improved phytoremediation capacity (en)
Title	Enhanced accumulation of Cadmium in Linum ussitatissimum L. plants due to overproduction of metallothionein α-domain as a fusion to β-glucuronidase gene protein Enhanced accumulation of Cadmium in Linum ussitatissimum L. plants due to overproduction of metallothionein α-domain as a fusion to β-glucuronidase gene protein (en)
skos:prefLabel	Enhanced accumulation of Cadmium in Linum ussitatissimum L. plants due to overproduction of metallothionein α-domain as a fusion to β-glucuronidase gene protein Enhanced accumulation of Cadmium in Linum ussitatissimum L. plants due to overproduction of metallothionein α-domain as a fusion to β-glucuronidase gene protein (en)
skos:notation	RIV/26784246:_____/13:#0000793!RIV14-MSM-26784246
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM2678424601)
http://linked.open...iv/cisloPeriodika	112
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Větrovcová, Martina Smýkalová, Iva Horáček, Jiří Griga, Miroslav Švábová, Lenka Vrbová, Miroslava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Agritec Plant Research s.r.o.
http://linked.open...dnocenehoVysledku	72920
http://linked.open...ai/riv/idVysledku	RIV/26784246:_____/13:#0000793
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Linum usitatissimum; Cadmium; Flax; Linseed; Metallothionein; Phytoextraction (en)
http://linked.open.../riv/klicoveSlovo	Phytoextraction Metallothionein Linum usitatissimum Linseed Cadmium Flax
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[A074EEC50084]
http://linked.open...i/riv/nazevZdroje	Plant Cell Tissue and Organ Culture
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	2013
http://linked.open...iv/tvurceVysledku	Griga, Miroslav Horáček, Jiří Smýkalová, Iva Vrbová, Miroslava Švábová, Lenka Větrovcová, Martina
http://linked.open...ain/vavai/riv/wos	000315277300006
http://linked.open...n/vavai/riv/zamer	Study and utilization of biotechnological and molecular methods in genetic and breeding research of genus Pisum and Linum
issn	1573-5044
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s11240-012-0239-1

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software