About: Resolving browning during the establishment of explant cultures in Vicia faba L. for genetic transformation.

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Resolving browning during the establishment of explant cultures in Vicia faba L. for genetic transformation. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://acta.mendelu.cz/61/5/1279/
Description	Optimisation of two in vitro regeneration systems for low-tannine cultivars of faba bean based on culturing of shoot apices and cotyledonary nodes were provided by usage of various antioxidants - ascorbic acid, citric acid, glutathione and activated charcoal. In subsequent testing, the combined effects of antioxidants with transformation co-cultivation compounds acetosyringone and L-cysteine was studied. The application of antioxidants lead to decreased caullogenesis, citric acids treatments (50 mg.l-1) dramatically decreased necrotic response of explants. However, citric acid, used together with ascorbic acid completely inhibited shoot growth in shoot apex cultures. Glutathion evoked hyperhydricity of explants. Activated charcoal induced rooting on media which are commonly used for shoot proliferation. Combination of acetosyringone with antioxidants did not negatively influenced shoot proliferation, except of variant with ascorbic acid. Citric acid worked as the best and universal antioxidant in faba bean in vitro cultures utilizable and its use is recommended for faba bean genetic transformation experiments. Optimisation of two in vitro regeneration systems for low-tannine cultivars of faba bean based on culturing of shoot apices and cotyledonary nodes were provided by usage of various antioxidants - ascorbic acid, citric acid, glutathione and activated charcoal. In subsequent testing, the combined effects of antioxidants with transformation co-cultivation compounds acetosyringone and L-cysteine was studied. The application of antioxidants lead to decreased caullogenesis, citric acids treatments (50 mg.l-1) dramatically decreased necrotic response of explants. However, citric acid, used together with ascorbic acid completely inhibited shoot growth in shoot apex cultures. Glutathion evoked hyperhydricity of explants. Activated charcoal induced rooting on media which are commonly used for shoot proliferation. Combination of acetosyringone with antioxidants did not negatively influenced shoot proliferation, except of variant with ascorbic acid. Citric acid worked as the best and universal antioxidant in faba bean in vitro cultures utilizable and its use is recommended for faba bean genetic transformation experiments. (en)
Title	Resolving browning during the establishment of explant cultures in Vicia faba L. for genetic transformation. Resolving browning during the establishment of explant cultures in Vicia faba L. for genetic transformation. (en)
skos:prefLabel	Resolving browning during the establishment of explant cultures in Vicia faba L. for genetic transformation. Resolving browning during the establishment of explant cultures in Vicia faba L. for genetic transformation. (en)
skos:notation	RIV/26784246:_____/13:#0000791!RIV14-MZE-26784246
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QI91A229)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Klenotičová, Helena Smýkalová, Iva Griga, Miroslav Švábová, Lenka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Agritec Plant Research s.r.o.
http://linked.open...dnocenehoVysledku	102611
http://linked.open...ai/riv/idVysledku	RIV/26784246:_____/13:#0000791
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	acetosyringone; antioxidants; citric cid; co-cultivation agents; faba bean; organogenesis (en)
http://linked.open.../riv/klicoveSlovo	antioxidants faba bean acetosyringone citric cid co-cultivation agents organogenesis
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[F80A34D99051]
http://linked.open...i/riv/nazevZdroje	Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Application of conventional and molecular genetic approaches for the development of grain legumes resistant to viral and fungal pathogens and insect pests.
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	61
http://linked.open...iv/tvurceVysledku	Griga, Miroslav Klenotičová, Helena Smýkalová, Iva Švábová, Lenka
issn	1211-8516
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.11118/actaun201361051279

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 47 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software