About: A new real-time PCR assay for rapid identification of the S. aureus/MRSA strains

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About: A new real-time PCR assay for rapid identification of the S. aureus/MRSA strains Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://acta.mendelu.cz/pdf/actaun201361061785.pdf
Description	Ther was developed a new more rapid and reliable diagnostic method working on the RT-PCR platform for monitoring of MRSA/S. aureus. PCR of the S. aureus specific nuc gene sequence and the mecA gene sequence was utilised for this purpose. A collection of ten S. aureus/MRSA reference strains, fifteen genetically related non S. aureus reference strains and fifty-six environmental samples was employed for estimation of the assay performance and parameters. The classic selective cultivation approach with the biochemical test and agar disk diffusion test was accepted as reference diagnostic method. Our method featured with 100% sensitivity in comparison to reference method. The limit of detection allowed to identify from tens to hundreds copies of S. aureus/MRSA genome. Further, the RT-PCR assay featured with 100% inclusivity and 95% exclusivity at Cq value below 30. We consider our method as ideal for testing of individual suspected colonies, when the results can be acquired in less than 1.5 hour. Ther was developed a new more rapid and reliable diagnostic method working on the RT-PCR platform for monitoring of MRSA/S. aureus. PCR of the S. aureus specific nuc gene sequence and the mecA gene sequence was utilised for this purpose. A collection of ten S. aureus/MRSA reference strains, fifteen genetically related non S. aureus reference strains and fifty-six environmental samples was employed for estimation of the assay performance and parameters. The classic selective cultivation approach with the biochemical test and agar disk diffusion test was accepted as reference diagnostic method. Our method featured with 100% sensitivity in comparison to reference method. The limit of detection allowed to identify from tens to hundreds copies of S. aureus/MRSA genome. Further, the RT-PCR assay featured with 100% inclusivity and 95% exclusivity at Cq value below 30. We consider our method as ideal for testing of individual suspected colonies, when the results can be acquired in less than 1.5 hour. (en)
Title	A new real-time PCR assay for rapid identification of the S. aureus/MRSA strains A new real-time PCR assay for rapid identification of the S. aureus/MRSA strains (en)
skos:prefLabel	A new real-time PCR assay for rapid identification of the S. aureus/MRSA strains A new real-time PCR assay for rapid identification of the S. aureus/MRSA strains (en)
skos:notation	RIV/26784246:_____/13:#0000761!RIV14-MZE-26784246
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QJ1210284)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Manga, Ivan Vyletělová, Marcela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Agritec Plant Research s.r.o.
http://linked.open...dnocenehoVysledku	58838
http://linked.open...ai/riv/idVysledku	RIV/26784246:_____/13:#0000761
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	RT-PCR; MRSA; S. aureus; livestock (en)
http://linked.open.../riv/klicoveSlovo	livestock MRSA RT-PCR S. aureus
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[ED8701CF6720]
http://linked.open...i/riv/nazevZdroje	Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Implementation of methods for MRSA detection in meat of food animals and methods for effective measures against their spread in the food chain.
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	LXI
http://linked.open...iv/tvurceVysledku	Manga, Ivan Vyletělová, Marcela
issn	1211-8516
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.11118/actaun201361061785

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