About: Transgenic pea with improved tolerance to Pea Enation Mosaic Virus.

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About: Transgenic pea with improved tolerance to Pea Enation Mosaic Virus. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Pea Enation Mosaic Virus (PEMV) and Pea Seed-borne Mosaic Virus (PSbMV) represent very important economical risks for pea growers by 10-25% decrease of seed yield. In our study, the induction of virus resistance / tolerance in transgenic pea lines were developed by the introgression of full length as well as fragments of cDNA of the PEMV and PSbMV viral coat proteins which were cloned in sense and antisense orientations into pGreenII plasmid (John Innes Centre, Norwich, UK). The level of virus replication after mechanical inoculation was monitored by time-sequenced DAS-ELISA. In transgenic pea lines T3 with PSbMV coat protein fragments the concentration of virus RNA was after 14 days transiently enhanced (in average up to 125%), but after 28 days the mean of RNA concentration was 37% as compared to the control non-transgenic inoculated plants (100%). Pea Enation Mosaic Virus (PEMV) and Pea Seed-borne Mosaic Virus (PSbMV) represent very important economical risks for pea growers by 10-25% decrease of seed yield. In our study, the induction of virus resistance / tolerance in transgenic pea lines were developed by the introgression of full length as well as fragments of cDNA of the PEMV and PSbMV viral coat proteins which were cloned in sense and antisense orientations into pGreenII plasmid (John Innes Centre, Norwich, UK). The level of virus replication after mechanical inoculation was monitored by time-sequenced DAS-ELISA. In transgenic pea lines T3 with PSbMV coat protein fragments the concentration of virus RNA was after 14 days transiently enhanced (in average up to 125%), but after 28 days the mean of RNA concentration was 37% as compared to the control non-transgenic inoculated plants (100%). (en)
Title	Transgenic pea with improved tolerance to Pea Enation Mosaic Virus. Transgenic pea with improved tolerance to Pea Enation Mosaic Virus. (en)
skos:prefLabel	Transgenic pea with improved tolerance to Pea Enation Mosaic Virus. Transgenic pea with improved tolerance to Pea Enation Mosaic Virus. (en)
skos:notation	RIV/26784246:_____/09:#0000151!RIV10-MSM-26784246
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM2678424601)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Smýkal, Petr Horáček, Jiří Griga, Miroslav Švábová, Lenka
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Agritec Plant Research s.r.o.
http://linked.open...dnocenehoVysledku	346737
http://linked.open...ai/riv/idVysledku	RIV/26784246:_____/09:#0000151
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	coat protein, fragment cDNA, induction of resistance, DAS-ELISA (en)
http://linked.open.../riv/klicoveSlovo	DAS-ELISA coat protein fragment cDNA induction of resistance
http://linked.open...ontrolniKodProRIV	[47A9AC8DD6BB]
http://linked.open...i/riv/mistoVydani	České Budějovice
http://linked.open...vEdiceCisloSvazku	neuvedeno
http://linked.open...i/riv/nazevZdroje	White book genetically modified crops
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...v/pocetStranKnihy	96 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Griga, Miroslav Hanáček, Pavel Horáček, Jiří Navrátil, Milan Reinöhl, Vilém Smýkal, Petr Šafářová, Dana Švábová, Lenka
http://linked.open...n/vavai/riv/zamer	Study and utilization of biotechnological and molecular methods in genetic and breeding research of genus Pisum and Linum
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Biology Centre of the Academy of Sciences of the Czech Republic
https://schema.org/isbn	978-80-86668-05-3

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