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rdf:type
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Description
| - To increase the percentage of the transformed tissue explants after cocultivation with Agrobacterium has been monitoring the influence of the removal of the hypocotyl epidermis , the influence of different time cocutivation and various acetosyringone concentrations. Efficiency of transformation was compared on the basis of GUS exxpresion on explants after 3 weeks from cocultivation using image analysis. Based on the statistical evaluation of the results of image analysis was evaluated he concentration of 100mg/l acetosyringonu as the most effecient and time of duration cocultivation 10 minutes. Removal of the epidermis increased the percentage of transformed tissue on 11,75%.
- To increase the percentage of the transformed tissue explants after cocultivation with Agrobacterium has been monitoring the influence of the removal of the hypocotyl epidermis , the influence of different time cocutivation and various acetosyringone concentrations. Efficiency of transformation was compared on the basis of GUS exxpresion on explants after 3 weeks from cocultivation using image analysis. Based on the statistical evaluation of the results of image analysis was evaluated he concentration of 100mg/l acetosyringonu as the most effecient and time of duration cocultivation 10 minutes. Removal of the epidermis increased the percentage of transformed tissue on 11,75%. (en)
- Pro zvýšení procenta transformovaných pletiv na explantátech po kokultivaci s Agrobacteriem byl sledován vliv odstranění epidermis z hypokotylu, vliv různé doby kokultivace a různých koncentrací acetosyringonu. Efektivita transformace byla porovnávána na základě GUS zabarvení explantátů po 3 týdnech od kokultivace pomocí obrazové analýzy. Na základě statistického hodnocení výsledků obrazové analýzy byla vyhodnocena jako neefektivnější koncentrace 100mg/l acetosyringonu a doba trvání kokultivace 10 minut. Odstranění epidermis zvýšilo procento transformovaných pletiv o 11,75%. (cs)
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Title
| - Improved transformation methodology for aquirement of stably transformed flax (Linum usitatissumum L.).
- Improved transformation methodology for aquirement of stably transformed flax (Linum usitatissumum L.). (en)
- Zdokonalení transformační metodiky pro získání stabilně transformovaného lnu. (cs)
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skos:prefLabel
| - Improved transformation methodology for aquirement of stably transformed flax (Linum usitatissumum L.).
- Improved transformation methodology for aquirement of stably transformed flax (Linum usitatissumum L.). (en)
- Zdokonalení transformační metodiky pro získání stabilně transformovaného lnu. (cs)
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skos:notation
| - RIV/26784246:_____/08:#0000103!RIV09-MSM-26784246
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(1M06030), Z(MSM2678424601)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/26784246:_____/08:#0000103
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Agrobacterium- mediated transformation, acetosyringone, flax (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
| - MZLU, Brno, Česká Republika
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http://linked.open...i/riv/nazevZdroje
| - Funkční genomika a proteomika ve šlechtění rostlin.
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Griga, Miroslav
- Smýkal, Petr
- Vrbová, Miroslava
- Švábová, Lenka
- Vinklárková, Petr
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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number of pages
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http://purl.org/ne...btex#hasPublisher
| - MZLU, Brno, Česká Republika
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https://schema.org/isbn
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