About: Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm.     Goto   Sponge   NotDistinct   Permalink

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  • We have used two highly abundant retrotransposons, representative one of the major group of long terminal repeats (LTR) - containing retrotransposons of Ty3/gypsy-like class, Ogre and Cyclop, for development of molecular markers for pea cultivars genotyping. The aim of this study was to access retrotransposon based markers to evaluate the genetic diversity of pea (Pisum sativum L.). To test the utility and reliability of the method we have chosen 20 commercially used pea cultivars with well known pedegree. Due to the sequence knowledge, IRAP markers are better scorable and result in more accurate genetic distance estimations over the REMAP, ISSR and RAPD techniques. With the use of just two outward-facing primers annealing to LTR Ogre and Cyclop target sequences 56 and 43 polymorphic bands per cultivar and reaction were produced which clearly distinguished and separated 20 investigated pea cultivars into groups related to known pedegree.
  • We have used two highly abundant retrotransposons, representative one of the major group of long terminal repeats (LTR) - containing retrotransposons of Ty3/gypsy-like class, Ogre and Cyclop, for development of molecular markers for pea cultivars genotyping. The aim of this study was to access retrotransposon based markers to evaluate the genetic diversity of pea (Pisum sativum L.). To test the utility and reliability of the method we have chosen 20 commercially used pea cultivars with well known pedegree. Due to the sequence knowledge, IRAP markers are better scorable and result in more accurate genetic distance estimations over the REMAP, ISSR and RAPD techniques. With the use of just two outward-facing primers annealing to LTR Ogre and Cyclop target sequences 56 and 43 polymorphic bands per cultivar and reaction were produced which clearly distinguished and separated 20 investigated pea cultivars into groups related to known pedegree. (en)
  • Pomocí dvou vybraných retrotransposonů Ogre a Cyclop byla vyvinuta metoda vhodná pro studium genetické struktury genofondových sbírek harchu. Výhoda této IRAP techniky ve srovnání v jinými je vysoká specificita a zisk dostatečného množství informací postačujících k odlišení i blízce příbuzných odrůd hrachu (cs)
Title
  • Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm.
  • Využitelnost vysoce kopiových retrotransposonových elementů Ogre a cyclop pro molekulární genotypizaci kolekce hrachu. (cs)
  • Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm. (en)
skos:prefLabel
  • Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm.
  • Využitelnost vysoce kopiových retrotransposonových elementů Ogre a cyclop pro molekulární genotypizaci kolekce hrachu. (cs)
  • Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm. (en)
skos:notation
  • RIV/26784246:_____/04:#0000005!RIV07-MSM-26784246
http://linked.open.../vavai/riv/strany
  • 326
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM2678424601)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 591837
http://linked.open...ai/riv/idVysledku
  • RIV/26784246:_____/04:#0000005
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • pea, genetic diversity, fingerprinting, retrotransposon (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [12DCB6513873]
http://linked.open...v/mistoKonaniAkce
  • Tulln , Rakousko
http://linked.open...i/riv/mistoVydani
  • Vienna, Rakousko
http://linked.open...i/riv/nazevZdroje
  • Genetic Variation for Plant Breeding
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Dostálová, Radmila
  • Griga, Miroslav
  • Hýbl, Miroslav
  • Smýkal, Petr
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • BOKU University, Tull
https://schema.org/isbn
  • 3-900962-56-1
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