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| rdf:type
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| Description
| - Acetylcholinesterase (AChE) is an enzyme playing a pivotal role in cholinergic neurotransmission. It is inhibited by a wide group of neurotoxic compounds. Owing to the practical significance, pesticides (e.g. carbaryl, dichlorvos, dimethoate) nerve agents (e.g. sarin, soman, tabun, VX) natural toxins (e.g. aflatoxins from Aspergillus flavus) and drugs (e.g. Alzheimer´s disease drugs galantamine, rivastigmine, donepezil) can be example as the AChE inhibitors. The neurotoxins can be easily assayed by devices containing AChE as a recognition element. Several colorimetric dipsticks and biosensors are typical tools containing AChE. The reported experiment is devoted to the construction of dipstick with immobilized AChE and indoxylacetate respective 2,5-dichlorophenol acetate as substrates. Original idea is to construct devices providing a contrast coloration comparing to the commercially available devices based on color change white to yellow as they use Ellman´s reagent. AChE was immobilized as a membrane stabilized by gelatin, glutaraldehyde and albumin, or cyclodextrins on cellulose, wax film, cotton gauze or alumina covered by silicagel. The immobilization procedure was optimized. After the optimization, the prepared dipsticks were tested for a long term stability and suitability for assay of neurotoxic compounds. We proved good stability with no relevant decay of the immobilized AChE activity when dipstick stored at laboratory temperature for one month. We assayed nerve agents (sarin, soman, VX), paraoxon and neostigmine as representative neurotoxins. When assessed the coloration by a naked eye, the limit of detection was approximately 100 nmol/l for the tested compounds. We infer suitability of the constructed dipsticks for fast and low cost field assay.
- Acetylcholinesterase (AChE) is an enzyme playing a pivotal role in cholinergic neurotransmission. It is inhibited by a wide group of neurotoxic compounds. Owing to the practical significance, pesticides (e.g. carbaryl, dichlorvos, dimethoate) nerve agents (e.g. sarin, soman, tabun, VX) natural toxins (e.g. aflatoxins from Aspergillus flavus) and drugs (e.g. Alzheimer´s disease drugs galantamine, rivastigmine, donepezil) can be example as the AChE inhibitors. The neurotoxins can be easily assayed by devices containing AChE as a recognition element. Several colorimetric dipsticks and biosensors are typical tools containing AChE. The reported experiment is devoted to the construction of dipstick with immobilized AChE and indoxylacetate respective 2,5-dichlorophenol acetate as substrates. Original idea is to construct devices providing a contrast coloration comparing to the commercially available devices based on color change white to yellow as they use Ellman´s reagent. AChE was immobilized as a membrane stabilized by gelatin, glutaraldehyde and albumin, or cyclodextrins on cellulose, wax film, cotton gauze or alumina covered by silicagel. The immobilization procedure was optimized. After the optimization, the prepared dipsticks were tested for a long term stability and suitability for assay of neurotoxic compounds. We proved good stability with no relevant decay of the immobilized AChE activity when dipstick stored at laboratory temperature for one month. We assayed nerve agents (sarin, soman, VX), paraoxon and neostigmine as representative neurotoxins. When assessed the coloration by a naked eye, the limit of detection was approximately 100 nmol/l for the tested compounds. We infer suitability of the constructed dipsticks for fast and low cost field assay. (en)
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| Title
| - ACETYLCHOLINESTERASE BASED COLORIMETRIC DIPSTICKS FOR FAST ASSAY OF NEUROTOXINS
- ACETYLCHOLINESTERASE BASED COLORIMETRIC DIPSTICKS FOR FAST ASSAY OF NEUROTOXINS (en)
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| skos:prefLabel
| - ACETYLCHOLINESTERASE BASED COLORIMETRIC DIPSTICKS FOR FAST ASSAY OF NEUROTOXINS
- ACETYLCHOLINESTERASE BASED COLORIMETRIC DIPSTICKS FOR FAST ASSAY OF NEUROTOXINS (en)
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| skos:notation
| - RIV/25656180:_____/11:#0000015!RIV13-MPO-25656180
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/25656180:_____/11:#0000015
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Acetylcholinesterase, enzyme playing, nerve agents, pesticides, natural toxins (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Pohanka, Doc. RNDr. PhD, Miroslav
- Povrazník, Ing., Jozef
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