About: Occurrence of ochratoxin A and citrinin in Czech cereals and comparison of two HPLC methods for ochratoxin A detection     Goto   Sponge   NotDistinct   Permalink

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  • The aims of the study were to obtain information about the occurrence of ochratoxin A (OTA) and citrinin (CIT) in cereals harvested in the Czech Republic and to compare two analytical procedures for detecting OTA. A total of 34 cereal samples, including two matrix reference materials (R-Biopharm, Germany), were analysed. The results were compared with the limit for raw cereal grains used as a foodstuff according to Commission Regulation No. 1881/2006, which allows a maximum OTA level of 5 mu g kg-1. Compared were two methods based on the high-performance liquid chromatography principle, one using the immunoaffinity columns OchraTest (VICAM) and the second based on solvent partition (PART), both followed by fluorescence detection. The highest OTA contents were found in two barley samples. According to the method employed, the results for the first sample (malting barley) were VICAM = 31.43 mu g kg-1 and PART = 44.74 mu g kg-1. For the second sample (feeding barley) they were VICAM = 48.63 mu g kg-1 and PART = 34.40 mu g kg-1. Two samples of bread wheat had an OTA content approaching the legal limit (VICAM = 4.71 mu g kg-1 and PART = 6.03 mu g kg-1; VICAM = 4.12 mu g kg-1 and PART = 3.95 mu g kg-1). CIT was analysed using the PART method only, and its highest content (93.64 mu g kg-1) was found for the malting barley sample with high OTA content (44.74 mu g kg-1 as analysed using PART).
  • The aims of the study were to obtain information about the occurrence of ochratoxin A (OTA) and citrinin (CIT) in cereals harvested in the Czech Republic and to compare two analytical procedures for detecting OTA. A total of 34 cereal samples, including two matrix reference materials (R-Biopharm, Germany), were analysed. The results were compared with the limit for raw cereal grains used as a foodstuff according to Commission Regulation No. 1881/2006, which allows a maximum OTA level of 5 mu g kg-1. Compared were two methods based on the high-performance liquid chromatography principle, one using the immunoaffinity columns OchraTest (VICAM) and the second based on solvent partition (PART), both followed by fluorescence detection. The highest OTA contents were found in two barley samples. According to the method employed, the results for the first sample (malting barley) were VICAM = 31.43 mu g kg-1 and PART = 44.74 mu g kg-1. For the second sample (feeding barley) they were VICAM = 48.63 mu g kg-1 and PART = 34.40 mu g kg-1. Two samples of bread wheat had an OTA content approaching the legal limit (VICAM = 4.71 mu g kg-1 and PART = 6.03 mu g kg-1; VICAM = 4.12 mu g kg-1 and PART = 3.95 mu g kg-1). CIT was analysed using the PART method only, and its highest content (93.64 mu g kg-1) was found for the malting barley sample with high OTA content (44.74 mu g kg-1 as analysed using PART). (en)
Title
  • Occurrence of ochratoxin A and citrinin in Czech cereals and comparison of two HPLC methods for ochratoxin A detection
  • Occurrence of ochratoxin A and citrinin in Czech cereals and comparison of two HPLC methods for ochratoxin A detection (en)
skos:prefLabel
  • Occurrence of ochratoxin A and citrinin in Czech cereals and comparison of two HPLC methods for ochratoxin A detection
  • Occurrence of ochratoxin A and citrinin in Czech cereals and comparison of two HPLC methods for ochratoxin A detection (en)
skos:notation
  • RIV/25328859:_____/10:#0000573!RIV12-MSM-25328859
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM2532885901)
http://linked.open...iv/cisloPeriodika
  • 11
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 276178
http://linked.open...ai/riv/idVysledku
  • RIV/25328859:_____/10:#0000573
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • HPLC; mycotoxins; ochratoxin A; cereals (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [77AEB61DAB1A]
http://linked.open...i/riv/nazevZdroje
  • FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 27
http://linked.open...iv/tvurceVysledku
  • Denešová, Olga
  • Dohnal, Vlastimil
  • Polišenská, Ivana
  • Ježková, Alena
  • Jirsa, Ondřej
  • Pfohl-Leszkowicz, Annie
  • Hadjeba, Kheira
  • Macharáčková, P.
http://linked.open...ain/vavai/riv/wos
  • 000281731700005
http://linked.open...n/vavai/riv/zamer
issn
  • 1944-0049
number of pages
http://bibframe.org/vocab/doi
  • 10.1080/19440049.2010.485580
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