About: Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues

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About: Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cochliobolus sativus (anamorph: Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melanin biosynthesis pathway Brn1 locus. Specificity was tested against more than 30 fungal organisms associated with barley and wheat. Limit of detection was determined to be 0.001 ng for pure fungal DNA. The species-specific primers were capable of detecting the pathogen in planta, specifically from infected barley leaves and wheat roots and stem bases. (C) 2010 Published by Elsevier Ltd. Cochliobolus sativus (anamorph: Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melanin biosynthesis pathway Brn1 locus. Specificity was tested against more than 30 fungal organisms associated with barley and wheat. Limit of detection was determined to be 0.001 ng for pure fungal DNA. The species-specific primers were capable of detecting the pathogen in planta, specifically from infected barley leaves and wheat roots and stem bases. (C) 2010 Published by Elsevier Ltd. (en)
Title	Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues (en)
skos:prefLabel	Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues (en)
skos:notation	RIV/25328859:_____/10:#0000504!RIV11-MZE-25328859
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QH71213)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Spitzer, Tomáš Tvarůžek, Ludvík Matušinsky, Pavel Svačinová, Ivana Mikolášová, Renata
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Agrotest fyto, s.r.o.
http://linked.open...dnocenehoVysledku	289038
http://linked.open...ai/riv/idVysledku	RIV/25328859:_____/10:#0000504
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cereal diseases; molecular diagnostics; spot blotch (en)
http://linked.open.../riv/klicoveSlovo	molecular diagnostics cereal diseases spot blotch
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[72582541C0B9]
http://linked.open...i/riv/nazevZdroje	Crop Protection
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Creation of new spring barley genotypes characterized by complex resistance to leaf and spike diseases
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Spitzer, Tomáš Tvarůžek, Ludvík Matušinsky, Pavel Mikolášová, Renata Svačinová, Ivana Frei, Peter
http://linked.open...ain/vavai/riv/wos	000282560400015
issn	0261-2194
number of pages	6 (xsd:int)

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