About: In Vitro Chemotherapy of Pear Cultivars

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of the present study was to determine whether chemotherapy with ribavirin can be used to eliminate Apple stem pitting virus (ASPV) from in vitro plants of infected pear cultivars 'Astra', 'David' and 'Erika'. According to RT-PCR testing, selected field-grown trees of all pear cultivars were infected with ASPV. In vitro cultures were successfully multiplied on MS medium with BAP (6-benzylaminopurine). Ribavirin was used at 20 mg L-1 for virus elimination. Low phytotoxicity of ribavirin at 20 mg L-1 was observep only for the cultivar 'David'. Six of the twenty shoot tips of 'David' showed apex necrosis and died after transfer to regeneration medium. The success of chemotherapy after RT-PCR testing of ribavirin treated plants varied between 65.0% for cultivars 'Astra' and 'Erika' and 71.4% for cultivar 'David'. The results achieved and the relatively high percentage of virus free plants obtained after 6 months of in vitro cultivation confirm the effectiveness of ribavirin in eliminating ASPV in pear. The aim of the present study was to determine whether chemotherapy with ribavirin can be used to eliminate Apple stem pitting virus (ASPV) from in vitro plants of infected pear cultivars 'Astra', 'David' and 'Erika'. According to RT-PCR testing, selected field-grown trees of all pear cultivars were infected with ASPV. In vitro cultures were successfully multiplied on MS medium with BAP (6-benzylaminopurine). Ribavirin was used at 20 mg L-1 for virus elimination. Low phytotoxicity of ribavirin at 20 mg L-1 was observep only for the cultivar 'David'. Six of the twenty shoot tips of 'David' showed apex necrosis and died after transfer to regeneration medium. The success of chemotherapy after RT-PCR testing of ribavirin treated plants varied between 65.0% for cultivars 'Astra' and 'Erika' and 71.4% for cultivar 'David'. The results achieved and the relatively high percentage of virus free plants obtained after 6 months of in vitro cultivation confirm the effectiveness of ribavirin in eliminating ASPV in pear. (en)
Title	In Vitro Chemotherapy of Pear Cultivars In Vitro Chemotherapy of Pear Cultivars (en)
skos:prefLabel	In Vitro Chemotherapy of Pear Cultivars In Vitro Chemotherapy of Pear Cultivars (en)
skos:notation	RIV/25271121:_____/14:#0000583!RIV15-MSM-25271121
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM2527112101)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Sedlák, Jiří Paprštein, František
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	VÝZKUMNÝ A ŠLECHTITELSKÝ ÚSTAV OVOCNÁŘSKÝ HOLOVOUSY s.r.o.
http://linked.open...dnocenehoVysledku	21071
http://linked.open...ai/riv/idVysledku	RIV/25271121:_____/14:#0000583
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ASPV; ELISA; PCR; virus detection; virus elimination; virus infection (en)
http://linked.open.../riv/klicoveSlovo	virus detection ELISA PCR virus infection virus elimination ASPV
http://linked.open...ontrolniKodProRIV	[5E757FE237F7]
http://linked.open...v/mistoKonaniAkce	Nelson, New Zealand
http://linked.open...i/riv/mistoVydani	Leuven
http://linked.open...i/riv/nazevZdroje	Acta Horticulturae
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Paprštein, František Sedlák, Jiří Talácko, L.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2012-03-25 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Research of apples and pears aimed at fruit quality and effectiveness of cultivation
issn	0567-7572
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	ISHS
https://schema.org/isbn	9789462610361

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