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Description
| - Fire blight, caused by Erwinia amylovora, is an infectious disease affecting pome fruit and other members of the rose family. It is a serious concern to producers of apples and pears in the Czech Republic. Under optimal conditions, it can seriously damage an entire apple or pear orchard in a growing season. Resistance of old local pome fruit cultivars to E. amylovora was tested in an in vitro culture assay. Four pear cultivars (´Belle de Bois´, ´Jakubka ceska´, ´Doynne de Merode´, ´Solanka´) and two apple cultivars (´Boiken´, ´Nonnetit´) were selected for testing. A verified isolate of E. amylovora (Ea 8/95) isolated from necrotic tissues of hawthorn (Crataegus x monogyna) was used in tests. The apical tip of the shoot was removed with scissors and two concentrations of the pathogen (103 and 106 cfu/ml) were applied on the cut surface. Based on the results, ´Solanka´ was the most resistant and ´Doynne de Merode´ the most susceptible of the selected pear cultivars. Concerning apple, two cultivars ´Boiken´ and ´Nonnetit´ were tested. In this test, cultivar ´Boiken´ was three times more susceptible than ´Nonnetit´. ´Nonnetit´ showed minor symptoms of necrosis 9 days after inoculation. Preliminary results from inoculation of pear and apple shoot tips maintained in tissue culture with E. amylovora was a useful method to test fire blight resistance in pome fruit cultivars. The obtained results are preliminary and evaluation of additional cultivars will be carried out in following years
- Fire blight, caused by Erwinia amylovora, is an infectious disease affecting pome fruit and other members of the rose family. It is a serious concern to producers of apples and pears in the Czech Republic. Under optimal conditions, it can seriously damage an entire apple or pear orchard in a growing season. Resistance of old local pome fruit cultivars to E. amylovora was tested in an in vitro culture assay. Four pear cultivars (´Belle de Bois´, ´Jakubka ceska´, ´Doynne de Merode´, ´Solanka´) and two apple cultivars (´Boiken´, ´Nonnetit´) were selected for testing. A verified isolate of E. amylovora (Ea 8/95) isolated from necrotic tissues of hawthorn (Crataegus x monogyna) was used in tests. The apical tip of the shoot was removed with scissors and two concentrations of the pathogen (103 and 106 cfu/ml) were applied on the cut surface. Based on the results, ´Solanka´ was the most resistant and ´Doynne de Merode´ the most susceptible of the selected pear cultivars. Concerning apple, two cultivars ´Boiken´ and ´Nonnetit´ were tested. In this test, cultivar ´Boiken´ was three times more susceptible than ´Nonnetit´. ´Nonnetit´ showed minor symptoms of necrosis 9 days after inoculation. Preliminary results from inoculation of pear and apple shoot tips maintained in tissue culture with E. amylovora was a useful method to test fire blight resistance in pome fruit cultivars. The obtained results are preliminary and evaluation of additional cultivars will be carried out in following years (en)
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Title
| - Testing of Resistance to Erwinia amylovora in an In Vitro Culture Assay
- Testing of Resistance to Erwinia amylovora in an In Vitro Culture Assay (en)
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skos:prefLabel
| - Testing of Resistance to Erwinia amylovora in an In Vitro Culture Assay
- Testing of Resistance to Erwinia amylovora in an In Vitro Culture Assay (en)
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skos:notation
| - RIV/25271121:_____/11:#0000191!RIV13-MZE-25271121
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/25271121:_____/11:#0000191
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - fire blight; bacterium; apple; pear; infection (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Korba, Josef
- Paprštein, František
- Sedlák, Jiří
- Šillerová, Jana
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issn
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number of pages
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