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Description
| - The objective of this study was to investigate the possibility of optimizing routine tissue culture methods to proliferate two sweet cherry cultivars Karešova and Rivan. Shoot tips of two genotypes were successfully established in vitro. Six proliferation MS media containing 1, 2 and 4 mg/L BAP (6-benzylaminopurine), 0.5 and 1 mg/L TDZ (thidiazuron) or 10 mg/L 2iP (6-(g,g-dimethylallylamino) purine) were tested. The highest proliferation rate (3.0 +- 0.1) was obtained for Rivan on MS medium containing 2 mg/L BAP. In the case of cultivar Karešova, any of the cytokinins tested did not promote satisfactory proliferation. The highest proliferation rate (1.6) achieved on MS medium with 2 mg/L 2iP is not sufficient for larger scale in vitro shoot production. It was proved that different genotypes of sweet cherry do not respond in the same way during proliferation in vitro. Future research and testing of other media and plant growth regulators will be carried out.
- The objective of this study was to investigate the possibility of optimizing routine tissue culture methods to proliferate two sweet cherry cultivars Karešova and Rivan. Shoot tips of two genotypes were successfully established in vitro. Six proliferation MS media containing 1, 2 and 4 mg/L BAP (6-benzylaminopurine), 0.5 and 1 mg/L TDZ (thidiazuron) or 10 mg/L 2iP (6-(g,g-dimethylallylamino) purine) were tested. The highest proliferation rate (3.0 +- 0.1) was obtained for Rivan on MS medium containing 2 mg/L BAP. In the case of cultivar Karešova, any of the cytokinins tested did not promote satisfactory proliferation. The highest proliferation rate (1.6) achieved on MS medium with 2 mg/L 2iP is not sufficient for larger scale in vitro shoot production. It was proved that different genotypes of sweet cherry do not respond in the same way during proliferation in vitro. Future research and testing of other media and plant growth regulators will be carried out. (en)
- Cílem této práce bylo studium možnosti množení dvou odrůd třešně Karešova a Rivan v in vitro kultuře. Růstové vrcholy obou genotypů byly úspěšně zavedeny do in vitro kultury. Bylo zkoumáno šest multiplikačních MS médií s obsahem 1, 2 a 4 mg/L BAP (6-benzylaminopurin), 0,5 a 1 mg/L TDZ (thidiazuron) nebo 10 mg/L 2iP (6-(g,g-dimethylallylamino) purin). Nejvyšší multiplikační koeficient (3,0 +- 0,1) byl obdržen pro odrůdu Rivan na MS médiu s obsahem 2 mg/L BAP. V případě odrůdy Karešova nevyvolal žádný z použitých cytokininů dostatečně vysokou multiplikaci. Nejvyšší dosažený multiplikační koeficient (1,6) z varianty MS media se 2 mg/L 2iP je příliš nízký pro produkci in vitro výhonů ve větším měřítku. Bylo potvrzeno, že různé genotypy třešně reagují odlišně v průběhu růstu a množení v in vitro kultuře. Z tohoto důvodu bude prováděno další testování růstových médií a regulátorů růstu. (cs)
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Title
| - In Vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan
- In Vitro množení odrůd třešně Karešova a Rivan (cs)
- In Vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan (en)
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skos:prefLabel
| - In Vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan
- In Vitro množení odrůd třešně Karešova a Rivan (cs)
- In Vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan (en)
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skos:notation
| - RIV/25271121:_____/08:9P002212!RIV09-MZE-25271121
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/25271121:_____/08:9P002212
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Prunus avium; micropropagation; culture initiation; multiplication; cytokinins (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Paprštein, František
- Sedlák, Jiří
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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