About: In Vitro Propagation of the P-HL Dwarfing Sweet Cherry Rootstocks

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We have developed a rapid shoot multiplication procedure for in vitro propagation of dwarfing P-HL rootstocks (P-HL-A, P-HL-B, P-HL-C) for sweet cherries selected in RBIP Holovousy. Shoot tips were successfully established in vitro and micropropagated on Murashige and Skoog (MS) based media containing different concentration of 6-benzylaminopurine (BAP). Multiplication rates varied depending on the rootstock and concentration of BAP. The highest multiplication rate was obtained for P-HL-A rootstock that produced 10.9 +-0.5 shoots (longer than 10 mm) on MS medium containing 1.5 mg/L BAP. The lowest multiplication rate was obtained for P-HL-B producing only 2.0 +- 0.3 shoots on MS medium containing 0.2 mg/L BAP. Higher concentration of BAP 2 mg/L was deleterious to shoot growth and multiplication rate. Effects of antibiotic cefotaxime at 200 mg/L on eliminating bacterial contamination were evaluated during in vitro multiplication. Also reported is the in vitro rooting. We have developed a rapid shoot multiplication procedure for in vitro propagation of dwarfing P-HL rootstocks (P-HL-A, P-HL-B, P-HL-C) for sweet cherries selected in RBIP Holovousy. Shoot tips were successfully established in vitro and micropropagated on Murashige and Skoog (MS) based media containing different concentration of 6-benzylaminopurine (BAP). Multiplication rates varied depending on the rootstock and concentration of BAP. The highest multiplication rate was obtained for P-HL-A rootstock that produced 10.9 +-0.5 shoots (longer than 10 mm) on MS medium containing 1.5 mg/L BAP. The lowest multiplication rate was obtained for P-HL-B producing only 2.0 +- 0.3 shoots on MS medium containing 0.2 mg/L BAP. Higher concentration of BAP 2 mg/L was deleterious to shoot growth and multiplication rate. Effects of antibiotic cefotaxime at 200 mg/L on eliminating bacterial contamination were evaluated during in vitro multiplication. Also reported is the in vitro rooting. (en)
Title	In Vitro Propagation of the P-HL Dwarfing Sweet Cherry Rootstocks In Vitro Propagation of the P-HL Dwarfing Sweet Cherry Rootstocks (en)
skos:prefLabel	In Vitro Propagation of the P-HL Dwarfing Sweet Cherry Rootstocks In Vitro Propagation of the P-HL Dwarfing Sweet Cherry Rootstocks (en)
skos:notation	RIV/25271121:_____/08:#0000110!RIV10-MSM-25271121
http://linked.open...avai/riv/aktivita	V
http://linked.open...avai/riv/aktivity	V
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Sedlák, Jiří Paprštein, František Erbenová, Marie
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	VÝZKUMNÝ A ŠLECHTITELSKÝ ÚSTAV OVOCNÁŘSKÝ HOLOVOUSY s.r.o.
http://linked.open...dnocenehoVysledku	371789
http://linked.open...ai/riv/idVysledku	RIV/25271121:_____/08:#0000110
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Prunus avium; in vitro multiplication; Cefotaxime; 6-benzylaminopurine (en)
http://linked.open.../riv/klicoveSlovo	6-benzylaminopurine Prunus avium Cefotaxime in vitro multiplication
http://linked.open...ontrolniKodProRIV	[143044BFFF15]
http://linked.open...v/mistoKonaniAkce	Bursa
http://linked.open...i/riv/mistoVydani	Leuven
http://linked.open...i/riv/nazevZdroje	Acta Horticulturae
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Paprštein, František Sedlák, Jiří Erbenová, Marie
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open...ain/vavai/riv/wos	000260482900059
http://linked.open.../riv/zahajeniAkce	2005-06-06 (xsd:date)
issn	0567-7572
number of pages	5 (xsd:int)
http://purl.org/ne...btex#hasPublisher	International Society Horticultural Science
https://schema.org/isbn	978-90-6605-551-3

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