About: Proliferation and cold hardening of in vitro grown apple shoot tips

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Shoot tips of apple cv. Rubin were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg /L BAP and 0.1 mg /L IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg/ L BAP, which produced 4.2 +/- 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. Rubin were cultivated for 4 weeks at low temperature (4 degrees C) and short photoperiod (8/16-h - light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. The lethal temperature (LT) was 13.0 degrees C for cold hardened and -6.5 degrees C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of cv. Rubin was 78% after thawing in 40 degrees C water. Shoot tips of apple cv. Rubin were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg /L BAP and 0.1 mg /L IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg/ L BAP, which produced 4.2 +/- 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. Rubin were cultivated for 4 weeks at low temperature (4 degrees C) and short photoperiod (8/16-h - light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. The lethal temperature (LT) was 13.0 degrees C for cold hardened and -6.5 degrees C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of cv. Rubin was 78% after thawing in 40 degrees C water. (en)
Title	Proliferation and cold hardening of in vitro grown apple shoot tips Proliferation and cold hardening of in vitro grown apple shoot tips (en)
skos:prefLabel	Proliferation and cold hardening of in vitro grown apple shoot tips Proliferation and cold hardening of in vitro grown apple shoot tips (en)
skos:notation	RIV/25271121:_____/06:0R000945!RIV10-MZE-25271121
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QC1052)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Sedlák, Jiří Paprštein, František
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	VÝZKUMNÝ A ŠLECHTITELSKÝ ÚSTAV OVOCNÁŘSKÝ HOLOVOUSY s.r.o.
http://linked.open...dnocenehoVysledku	495488
http://linked.open...ai/riv/idVysledku	RIV/25271121:_____/06:0R000945
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	micropropagation; germplasm; encapsulation; cryopreservation; freezing (en)
http://linked.open.../riv/klicoveSlovo	cryopreservation freezing germplasm encapsulation micropropagation
http://linked.open...ontrolniKodProRIV	[9D9BE7457F9D]
http://linked.open...v/mistoKonaniAkce	Debrecen
http://linked.open...i/riv/mistoVydani	Leuven
http://linked.open...i/riv/nazevZdroje	Acta Horticulturae
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	New method of conservation of fruit crop gene resources.
http://linked.open...UplatneniVysledku	2006
http://linked.open...iv/tvurceVysledku	Zámečník, Jiří Paprštein, František Sedlák, Jiří Bilavčík, Alois
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open...ain/vavai/riv/wos	000245292600066
http://linked.open.../riv/zahajeniAkce	2004-09-12 (xsd:date)
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	International Society for Horticultural Science
https://schema.org/isbn	978-90-6605-719-7

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