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Description
  • In this study we evaluated applicability of a new cryopreservation method for conservation of hop germplasm. 35 cultivars of Humulus lupulus and one clone of Humulus japonicus were introduced into in vitro conditions, evaluated on viral infection, cryopreserved and recovered for tests of genotype stability. Altogether 72 independent experiments were performed for verification of the cryopreservation method. The average explant post-thaw recovery of all evaluated accessions was 38 % whereas 72 % accessions had post-thaw recovery higher than 30 %. The results guaranteed successful recovery of all cryopreserved cultivars. Tests of genotype stability by SSR and STS analyses did not detect any changes due to cryopreservation.
  • In this study we evaluated applicability of a new cryopreservation method for conservation of hop germplasm. 35 cultivars of Humulus lupulus and one clone of Humulus japonicus were introduced into in vitro conditions, evaluated on viral infection, cryopreserved and recovered for tests of genotype stability. Altogether 72 independent experiments were performed for verification of the cryopreservation method. The average explant post-thaw recovery of all evaluated accessions was 38 % whereas 72 % accessions had post-thaw recovery higher than 30 %. The results guaranteed successful recovery of all cryopreserved cultivars. Tests of genotype stability by SSR and STS analyses did not detect any changes due to cryopreservation. (en)
Title
  • Progress in the Czech hop Germplasm Cryoconservation
  • Progress in the Czech hop Germplasm Cryoconservation (en)
skos:prefLabel
  • Progress in the Czech hop Germplasm Cryoconservation
  • Progress in the Czech hop Germplasm Cryoconservation (en)
skos:notation
  • RIV/14864347:_____/11:#0000354!RIV12-MZE-14864347
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QF3039)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 224470
http://linked.open...ai/riv/idVysledku
  • RIV/14864347:_____/11:#0000354
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cryoconservation and cryopreservation; Humulus lupulus L.; liquid nitrogen (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [1E82128AD94A]
http://linked.open...v/mistoKonaniAkce
  • Leuven, Belgie
http://linked.open...i/riv/mistoVydani
  • Leuven, Belgie
http://linked.open...i/riv/nazevZdroje
  • Proceedings of the First International Symposium on Cryopreservation in Horticultural Species. Acta Horticularae 908
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http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Faltus, Miloš
  • Nesvadba, Vladimír
  • Svoboda, Petr
  • Zámečník, J.
  • Patzak, Josef
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
issn
  • 0567-7572
number of pages
http://purl.org/ne...btex#hasPublisher
  • International Society for Horticultural Science
https://schema.org/isbn
  • 978-90-6605-470-7
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