About: Gene specific molecular markers for hop (Humulus lupulus L.)

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.actahort.org/books/848/848_7.htm
Description	A new trend in molecular methods is to develop new molecular markers in specific sequences, near or within structural genes. Recently, markers in intron, 5’ and 3’UTR were identified in several hop genes. Increasing sequence information of hop genes in Genebank provides additional opportunities to develop new gene specific molecular markers. In this study, we report new efficient and reliable expressed sequence tag microsatellite repeat (EST-SSR) molecular markers. We used forty primer pairs for twenty-nine gene specific sequences. We evaluated them for identification and clustering in eight hops cultivars. Gene-specific based clustering was compared to clustering based on SSR, AFLP markers and on a combination of molecular markers. These molecular markers are ideal for DNA fingerprinting, for control of authenticity and purity of varieties, for evaluation of genetic diversity in genetic resources, etc. A new trend in molecular methods is to develop new molecular markers in specific sequences, near or within structural genes. Recently, markers in intron, 5’ and 3’UTR were identified in several hop genes. Increasing sequence information of hop genes in Genebank provides additional opportunities to develop new gene specific molecular markers. In this study, we report new efficient and reliable expressed sequence tag microsatellite repeat (EST-SSR) molecular markers. We used forty primer pairs for twenty-nine gene specific sequences. We evaluated them for identification and clustering in eight hops cultivars. Gene-specific based clustering was compared to clustering based on SSR, AFLP markers and on a combination of molecular markers. These molecular markers are ideal for DNA fingerprinting, for control of authenticity and purity of varieties, for evaluation of genetic diversity in genetic resources, etc. (en)
Title	Gene specific molecular markers for hop (Humulus lupulus L.) Gene specific molecular markers for hop (Humulus lupulus L.) (en)
skos:prefLabel	Gene specific molecular markers for hop (Humulus lupulus L.) Gene specific molecular markers for hop (Humulus lupulus L.) (en)
skos:notation	RIV/14864347:_____/09:#0000376!RIV12-MZE-14864347
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA521/08/0740), P(QH81052), Z(AV0Z50510513)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Patzak, Josef
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Chmelařský institut s.r.o.
http://linked.open...dnocenehoVysledku	316054
http://linked.open...ai/riv/idVysledku	RIV/14864347:_____/09:#0000376
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	expressed sequence tag (EST); single nucleotide polymorphism (SNP); microsatellite repeat; simple sequence repeat (SSR); insertion/deletion (indel); cluster analysis (en)
http://linked.open.../riv/klicoveSlovo	insertion/deletion (indel) microsatellite repeat cluster analysis simple sequence repeat (SSR) single nucleotide polymorphism (SNP) expressed sequence tag (EST)
http://linked.open...ontrolniKodProRIV	[1B28DC5D1BFB]
http://linked.open...v/mistoKonaniAkce	Ghent, Belgie
http://linked.open...i/riv/mistoVydani	Leuven, Belgie
http://linked.open...i/riv/nazevZdroje	Proceedings of the Second International Humulus Symposium, Acta Horticulturae 848
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular analysis of transcription factors of hop (H. lupulus L.) in relation to lupulin biosynthesis Development of molecular-genetic markers for modern breeding and gene engineering of hop (Humulus lupulus) based on the system of genomic and expression libraries.
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Matoušek, Jaroslav Patzak, Josef
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2008-09-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny
issn	0567-7572
number of pages	8 (xsd:int)
http://purl.org/ne...btex#hasPublisher	International Society for Horticultural Science
https://schema.org/isbn	9789066057227

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