About: Functional analysis of hop (Humulus lupulus L.) regulatory factors from bZIP and bHLH families in transient expression systems

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About: Functional analysis of hop (Humulus lupulus L.) regulatory factors from bZIP and bHLH families in transient expression systems Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Functional analyses of cloned hop regulatory factors (TF) HlbZIP1, HlbZIP2 and HlbHLH1 were performed in transient expression system using N. benthamiana leaf co-infiltration with A. tumefaciens containing TF and three hop promoters fused to GUS reference gene. bZIPs and bHLH genes were combined with HlMyb1, l-HlMyb3, s-HlMyb3 TFs to check possible interactions. Both HlbZIPs and HlBHLH1 activated mainly promoters of chs_H1 and omt1 (Pomt1) genes, while the response of vps promoter to cloned hop TFs was very low. It was found that HlMyb1 activates strongly Pomt1 and could be thus useful in %22myb biotechnology%22 to modify regulation of this gene in transgenic hop. Proceedings of the Scientific Commission IHGC, 21-25. June 2009, León, Spain, p. 44-48. ISSN 1814-2192. Functional analyses of cloned hop regulatory factors (TF) HlbZIP1, HlbZIP2 and HlbHLH1 were performed in transient expression system using N. benthamiana leaf co-infiltration with A. tumefaciens containing TF and three hop promoters fused to GUS reference gene. bZIPs and bHLH genes were combined with HlMyb1, l-HlMyb3, s-HlMyb3 TFs to check possible interactions. Both HlbZIPs and HlBHLH1 activated mainly promoters of chs_H1 and omt1 (Pomt1) genes, while the response of vps promoter to cloned hop TFs was very low. It was found that HlMyb1 activates strongly Pomt1 and could be thus useful in %22myb biotechnology%22 to modify regulation of this gene in transgenic hop. Proceedings of the Scientific Commission IHGC, 21-25. June 2009, León, Spain, p. 44-48. ISSN 1814-2192. (en)
Title	Functional analysis of hop (Humulus lupulus L.) regulatory factors from bZIP and bHLH families in transient expression systems Functional analysis of hop (Humulus lupulus L.) regulatory factors from bZIP and bHLH families in transient expression systems (en)
skos:prefLabel	Functional analysis of hop (Humulus lupulus L.) regulatory factors from bZIP and bHLH families in transient expression systems Functional analysis of hop (Humulus lupulus L.) regulatory factors from bZIP and bHLH families in transient expression systems (en)
skos:notation	RIV/14864347:_____/09:#0000264!RIV10-MZE-14864347
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA521/08/0740), P(QH81052)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Krofta, Karel Patzak, Josef
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Chmelařský institut s.r.o.
http://linked.open...dnocenehoVysledku	315704
http://linked.open...ai/riv/idVysledku	RIV/14864347:_____/09:#0000264
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	hop (Humulus lupulus L.); transcription factors; transient expression assay; plant metabolome; prenylflavonoids (en)
http://linked.open.../riv/klicoveSlovo	plant metabolome transient expression assay hop (Humulus lupulus L.) transcription factors prenylflavonoids
http://linked.open...ontrolniKodProRIV	[007A8163A5D5]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular analysis of transcription factors of hop (H. lupulus L.) in relation to lupulin biosynthesis Development of molecular-genetic markers for modern breeding and gene engineering of hop (Humulus lupulus) based on the system of genomic and expression libraries.
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Krofta, Karel Matoušek, Jaroslav Patzak, Josef Kocábek, Tomáš Orctová, Lidmila

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