About: Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Novel fluoroalkyl chloroformates with 3 and 4 carbon atoms were investigated to immediate conversion of amino acids into hydrophobic derivatives in water-containing media. Derivatization conditions were extensively studied and optimized sample preparation protocols elaborated. More than 30 amino acids were treated with the particular reagent in isooctane by simply vortexing the reactive organic phase with a slightly basified aqueous medium containing pyridine or 3-picoline as a catalyst. Outstanding separation of nearly all components on 5% phenylmethylsilicone phase in gas chromatographic (GC) analysis with mass spectrometric (MS) or flame ionization detection (FID) required <10 min. Quantitation characteristics involving linearity in range of 0.1 to 100 nmol, MS limit of detection (LOD, 0.03 pmol - 20 pmol) and electron impact (EI) spectra and diagnostic SIM fragment ions of the derivatives are reported. The novel method is simple, robust and rapid, running in aqueous environment. Novel fluoroalkyl chloroformates with 3 and 4 carbon atoms were investigated to immediate conversion of amino acids into hydrophobic derivatives in water-containing media. Derivatization conditions were extensively studied and optimized sample preparation protocols elaborated. More than 30 amino acids were treated with the particular reagent in isooctane by simply vortexing the reactive organic phase with a slightly basified aqueous medium containing pyridine or 3-picoline as a catalyst. Outstanding separation of nearly all components on 5% phenylmethylsilicone phase in gas chromatographic (GC) analysis with mass spectrometric (MS) or flame ionization detection (FID) required <10 min. Quantitation characteristics involving linearity in range of 0.1 to 100 nmol, MS limit of detection (LOD, 0.03 pmol - 20 pmol) and electron impact (EI) spectra and diagnostic SIM fragment ions of the derivatives are reported. The novel method is simple, robust and rapid, running in aqueous environment. (en)
Title	Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis (en)
skos:prefLabel	Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis (en)
skos:notation	RIV/00843989:_____/08:00100618!RIV10-GA0-00843989
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/04/0192), P(GA303/06/1674), Z(AV0Z50070508)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Hušek, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice Ostrava
http://linked.open...dnocenehoVysledku	368246
http://linked.open...ai/riv/idVysledku	RIV/00843989:_____/08:00100618
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	amino acids; derivatization; pentafluoropropyl- and heptafluorobutyl chloroformates; gas chromatography (en)
http://linked.open.../riv/klicoveSlovo	derivatization gas chromatography amino acids pentafluoropropyl- and heptafluorobutyl chloroformates
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[6091F024E5BA]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	MULTICOMPLEX DIAGNOSTICS OF B-VITAMIN BIOMARKERS AND NUTRITIONAL STATUS IN PLASMA MICROSAMPLES USING A SIMPLE SAMPLE PRETREATMENT Design and synthesis of novel haloformates as advanced reagents in diagnostics and screening compounds of biological importance
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	1186
http://linked.open...iv/tvurceVysledku	Hušek, Petr Šimek, P. Hartvich, P. Zahradníčková, H.
http://linked.open...ain/vavai/riv/wos	000254884700034
http://linked.open...n/vavai/riv/zamer	Study of the regulation of insect organism, dynamics of insect populations and function of insects in ecosystems
issn	0021-9673
number of pages	10 (xsd:int)

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