| Attributes | Values |
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| rdf:type
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| Description
| - Citlivá a rychlá metoda byla vyvinuta pro souběžné stanovení kyseliny methylmalonové, celkového homocysteinu a příbuzných metabolitů kobalaminového a folátového metabolismu v séru kombinací plynové chromatografie s hmotnostní detekcí. Thioly v disulfidických a proteinových vazbách byly vyvázány působením 2,3-dimerkaptopropansulfonové kyseliny a derivatizovány heptafluorbutylchlorformiátem v jediném kroku. Nové redukční činidlo poskytlo podstatně čistší extrakty než při použití dithiothreitolu. Nový postup umožnil GC-MS analyzovat sérové metabolity, včetně cystathionu a SH-dipeptidů. Přesná kvantifikace 7 biomarkerů byla umožněna nasazením deuterovaných kalibrátorů. Detekční limit byl v oblasti 7-20 nmol/l, inter-assay přesnost 1,5-8,8% a zpětný výtěžek se pohyboval v rozmězí 83-103%. Metoda kombinované redukce a derivatizace se ukázala jako účinná pro diagnostiku a skríning sérových metabolitů (cs)
- A sensitive and rapid method has been developed for simultaneous determination of serum methylmalonic acid, total homocysteine and related metabolites of cobalamin and folate status by gas chromatography-mass spectrometry. Thiols bound in proteins and disulfides were released with 2,3-dimercaptopropanesulfonic acid, and after deproteinization, they were derivatized with heptafluorobutyl chloroformate in a single step. The novel reducing agent afforded much cleaner extracts than those using dithiothreitol. The new method enabled GC-MS screening of serum aminoacidic metabolites, including cystathionine and thiol-containing dipeptides. Accurate quantitation of seven biomarkers was accomplished by using deuterated internal standards; the detection limits ranged from 7 to 20 nmol/L, the between-day precision from 1.5 to 8.8%, and the recoveries were between 83 and 103%. The new combined reduction and derivatization procedure make the method efficient for diagnostics and screening of serum m
- A sensitive and rapid method has been developed for simultaneous determination of serum methylmalonic acid, total homocysteine and related metabolites of cobalamin and folate status by gas chromatography-mass spectrometry. Thiols bound in proteins and disulfides were released with 2,3-dimercaptopropanesulfonic acid, and after deproteinization, they were derivatized with heptafluorobutyl chloroformate in a single step. The novel reducing agent afforded much cleaner extracts than those using dithiothreitol. The new method enabled GC-MS screening of serum aminoacidic metabolites, including cystathionine and thiol-containing dipeptides. Accurate quantitation of seven biomarkers was accomplished by using deuterated internal standards; the detection limits ranged from 7 to 20 nmol/L, the between-day precision from 1.5 to 8.8%, and the recoveries were between 83 and 103%. The new combined reduction and derivatization procedure make the method efficient for diagnostics and screening of serum m (en)
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| Title
| - Gas Chromatographic-Mass Spectrometric Analysis of Biomarkers Related to Folate and Cobalamin Status in Human Serum after Dimercaptopropanesulfonate Reduction and Heptafluorobutyl Chloroformate Derivatization
- Gas Chromatographic-Mass Spectrometric Analysis of Biomarkers Related to Folate and Cobalamin Status in Human Serum after Dimercaptopropanesulfonate Reduction and Heptafluorobutyl Chloroformate Derivatization (en)
- Analýza biomarkerů folátového a kobalaminového metabolismu v lidském séru plynovou chromatografií s hmotnostní detekcí po redukci dimerkaptopropansulfonátem a derivatizaci chlormravenčanem heptafluorbutylnatým (cs)
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| skos:prefLabel
| - Gas Chromatographic-Mass Spectrometric Analysis of Biomarkers Related to Folate and Cobalamin Status in Human Serum after Dimercaptopropanesulfonate Reduction and Heptafluorobutyl Chloroformate Derivatization
- Gas Chromatographic-Mass Spectrometric Analysis of Biomarkers Related to Folate and Cobalamin Status in Human Serum after Dimercaptopropanesulfonate Reduction and Heptafluorobutyl Chloroformate Derivatization (en)
- Analýza biomarkerů folátového a kobalaminového metabolismu v lidském séru plynovou chromatografií s hmotnostní detekcí po redukci dimerkaptopropansulfonátem a derivatizaci chlormravenčanem heptafluorbutylnatým (cs)
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| skos:notation
| - RIV/00843989:_____/08:00015978!RIV09-GA0-00843989
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA303/06/1674), Z(AV0Z50070508)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00843989:_____/08:00015978
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - serum biomarkers; folate and cobalamin status; dimercaptopropanesulfonate for reduction; heptafluorobutyl chloroformate for derivatization; gas chromatography-mass spectrometry (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Hušek, Petr
- Šimek, P.
- Zahradníčková, H.
|
| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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