About: <A >Capillary GC Method Using Nitrogen Phosphorus Detection for Determination of Topiramate in Patients with Epilepsy

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A new capillary gas chromatographic assay with nitrogen phosphorus detection for the determination of topiramate in the serum was developed and validated. The sample procedure included a liquid-liquid extraction of 0.1 mL of alkalized sample with ethyl acetate. The organic solvent was evaporated, and flash methylation of topiramate and internal standard 5-(p-methylphenyl)-5-phenylhydantoin with trimethylanilinium hydroxide (0.07 mol L-1 in methanol) was performed. The structure of derivatization product was confirmed using gas chromatography-mass spectrometry. Validation experiments certified suitability of bioanalytical method in the monitoring of serum concentrations in epileptic patients. The limit of detection was found to be 1.69 mu mol L-1. The range of applicability and linearity was established from 4.35 to 69.62 mu mol L-1. The accuracy and precision reached acceptable values from 86.15 to 109.5% (recovery) respectively, values were from 2.19 to 11.03% (RSD). No interference w A new capillary gas chromatographic assay with nitrogen phosphorus detection for the determination of topiramate in the serum was developed and validated. The sample procedure included a liquid-liquid extraction of 0.1 mL of alkalized sample with ethyl acetate. The organic solvent was evaporated, and flash methylation of topiramate and internal standard 5-(p-methylphenyl)-5-phenylhydantoin with trimethylanilinium hydroxide (0.07 mol L-1 in methanol) was performed. The structure of derivatization product was confirmed using gas chromatography-mass spectrometry. Validation experiments certified suitability of bioanalytical method in the monitoring of serum concentrations in epileptic patients. The limit of detection was found to be 1.69 mu mol L-1. The range of applicability and linearity was established from 4.35 to 69.62 mu mol L-1. The accuracy and precision reached acceptable values from 86.15 to 109.5% (recovery) respectively, values were from 2.19 to 11.03% (RSD). No interference w (en) Vyvinuli jsme novou chromatografickou metodu s termionickým detektorem pro stanovení topiramátu v séru. Topiramát byl extrahován z 0,1 ml alkalizovaného séra etylacetátem. Organická vrstva se odpařila a provedla se metylace topiramátu a interního standardu 5-(p-methylphenyl)-5-phenylhydantoinu trimethylanilinium hydroxidem (0.07 mol L-1 v methanolu). Na potvrzení struktury derivatizovaných produktů jsme použili plynovou chromatografii s hmotnostní detekcí. Validace metody potvrdila její vhodnost pro monitorování sérových koncentrací topiramátu u epileptických pacientů. Limit detekce byl 1.69 mu mol L-1. Linearita byla stanovena od 4.35 do 69.62. Přesnost dosáhla akceptovatelných hodnot od 86.15 do 109.5% a variační koeficient byl od 2.19 do 11.03%. (cs)
Title	Capillary GC Method Using Nitrogen Phosphorus Detection for Determination of Topiramate in Patients with Epilepsy Capillary GC Method Using Nitrogen Phosphorus Detection for Determination of Topiramate in Patients with Epilepsy (en) Metoda kapalinové chromatografie s termionickou detekcí pro stanovení topiramátu u pacientů s epilepsií (cs)
skos:prefLabel	Capillary GC Method Using Nitrogen Phosphorus Detection for Determination of Topiramate in Patients with Epilepsy Capillary GC Method Using Nitrogen Phosphorus Detection for Determination of Topiramate in Patients with Epilepsy (en) Metoda kapalinové chromatografie s termionickou detekcí pro stanovení topiramátu u pacientů s epilepsií (cs)
skos:notation	RIV/00843989:_____/07:00014895!RIV09-MZ0-00843989
http://linked.open...avai/riv/aktivita	V Z
http://linked.open...avai/riv/aktivity	V, Z(MZ0FNHK2005)
http://linked.open...iv/cisloPeriodika	5/6
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Brozmanová, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice Ostrava
http://linked.open...dnocenehoVysledku	407790
http://linked.open...ai/riv/idVysledku	RIV/00843989:_____/07:00014895
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	gas chromatography; derivatization; topiramate (en)
http://linked.open.../riv/klicoveSlovo	derivatization gas chromatography topiramate
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[0C5E7FA60FEC]
http://linked.open...i/riv/nazevZdroje	Chromatographia
http://linked.open...in/vavai/riv/obor	FR
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	66
http://linked.open...iv/tvurceVysledku	Brozmanová, Hana Maláková, J. Palicka, V. Prochazkova, V. Vorisek, V.
http://linked.open...ain/vavai/riv/wos	000249712300009
http://linked.open...n/vavai/riv/zamer	Nové diagnostické indikátory a léčebné postupy v závislosti na různých obdobích života s důrazem na stáří
issn	0009-5893
number of pages	5 (xsd:int)

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