About: Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser- Induced Fluorescence Detection

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Doxorubicin (DOX) belongs to the group of anthracycline antibiotics with very effective anticancer properties. On the other hand, the cardiotoxic effects limit its application over the maximum cumulative dose. To overcome this obstacle, encapsulation of this drug into the protective nanotransporter such as apoferritin is beneficial. In this study, fluorescent behavior of DOX in various solvents was determined by fluorescence spectrometry, demonstrating the fluorescence quenching effect of water, which is often used as a solvent. It was found that by increasing the amount of the organic phase in the DOX solvent the dynamic quenching is significantly suppressed. Ethanol, acetonitrile and dimethyl sulfoxide were tested and the best linearity of the calibration curve was obtained when above 50 % of the solvent was present in the binary mixture with water. Moreover, pH influence on the DOX fluorescence was also observed within the range of 4–10. Two times higher fluorescence intensity was observed at pH Doxorubicin (DOX) belongs to the group of anthracycline antibiotics with very effective anticancer properties. On the other hand, the cardiotoxic effects limit its application over the maximum cumulative dose. To overcome this obstacle, encapsulation of this drug into the protective nanotransporter such as apoferritin is beneficial. In this study, fluorescent behavior of DOX in various solvents was determined by fluorescence spectrometry, demonstrating the fluorescence quenching effect of water, which is often used as a solvent. It was found that by increasing the amount of the organic phase in the DOX solvent the dynamic quenching is significantly suppressed. Ethanol, acetonitrile and dimethyl sulfoxide were tested and the best linearity of the calibration curve was obtained when above 50 % of the solvent was present in the binary mixture with water. Moreover, pH influence on the DOX fluorescence was also observed within the range of 4–10. Two times higher fluorescence intensity was observed at pH (en)
Title	Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser- Induced Fluorescence Detection Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser- Induced Fluorescence Detection (en)
skos:prefLabel	Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser- Induced Fluorescence Detection Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser- Induced Fluorescence Detection (en)
skos:notation	RIV/00216305:26620/14:PU112643!RIV15-MSM-26620___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP301/10/0356)
http://linked.open...iv/cisloPeriodika	21-22
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Konečná, Romana Ryvolová, Markéta Křížková, Soňa Nguyen, Hoai Viet
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	12326
http://linked.open...ai/riv/idVysledku	RIV/00216305:26620/14:PU112643
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Capillary electrophoresis, Fluorescence, Drug delivery, Doxorubicin, Apoferritin (en)
http://linked.open.../riv/klicoveSlovo	Apoferritin Capillary electrophoresis Doxorubicin Fluorescence Drug delivery
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[633E02E10A4A]
http://linked.open...i/riv/nazevZdroje	CHROMATOGRAPHIA
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of contribution of different DNA-damaging mechanisms to toxicity of cytostatics to human chemosensitive and chemoresistant neuroblastomas
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	77
http://linked.open...iv/tvurceVysledku	Křížková, Soňa Stiborová, Marie Vaculovičová, Markéta Blažková, Iva Nguyen, Hoai Viet Stanisavljevic, Maja Konečná, Romana
issn	0009-5893
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	26620

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