About: Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza

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About: Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Considerable efforts have been devoted to the development of rapid and sensitive methods allowing the detection of viral nucleic acid. We herein describe an assay for identification of a specific influenza sequence. The suggested method was based on isolation using paramagnetic particles coupled with electrochemical detection of isolated product. Peptide nucleic acid (PNA) was used as a probe for hybridization and identification of the influenza-derived specific sequence. The use of PNA can show numerous benefits: PNA probe is not degradable by enzymes and the duplex of PNA with RNA/DNA is more thermostable and more resistant to pH changes than DNA/DNA or RNA/ RNA duplexes. This PNA probe assay can be applied as a magnetically guidable tool for detection of DNA/RNA samples under different conditions. Considerable efforts have been devoted to the development of rapid and sensitive methods allowing the detection of viral nucleic acid. We herein describe an assay for identification of a specific influenza sequence. The suggested method was based on isolation using paramagnetic particles coupled with electrochemical detection of isolated product. Peptide nucleic acid (PNA) was used as a probe for hybridization and identification of the influenza-derived specific sequence. The use of PNA can show numerous benefits: PNA probe is not degradable by enzymes and the duplex of PNA with RNA/DNA is more thermostable and more resistant to pH changes than DNA/DNA or RNA/ RNA duplexes. This PNA probe assay can be applied as a magnetically guidable tool for detection of DNA/RNA samples under different conditions. (en)
Title	Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza (en)
skos:prefLabel	Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza (en)
skos:notation	RIV/00216305:26620/14:PU112580!RIV15-MSM-26620___
http://linked.open...avai/riv/aktivita	O P
http://linked.open...avai/riv/aktivity	O, P(ED1.1.00/02.0068)
http://linked.open...iv/cisloPeriodika	21-22
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Hynek, David Adam, Vojtěch Kizek, René Krejčová, Ludmila Guráň, Roman Nguyen, Hoai Viet
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	35716
http://linked.open...ai/riv/idVysledku	RIV/00216305:26620/14:PU112580
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Protein nucleic acid, Biosensor, Magneticparticle- based separation, Voltammetry (en)
http://linked.open.../riv/klicoveSlovo	Magneticparticle- based separation Protein nucleic acid Biosensor Voltammetry
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[5E65FAA1A0F5]
http://linked.open...i/riv/nazevZdroje	CHROMATOGRAPHIA
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	77
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Hynek, David Kizek, René Krejčová, Ludmila Nguyen, Hoai Viet Guráň, Roman
issn	0009-5893
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	26620

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