About: An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry     Goto   Sponge   NotDistinct   Permalink

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Description
  • This study was focused on the application of electrochemical methods for studying of bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after theapplication of cadmium and/or lead ions in four concentrations (25, 50, 75 and 150 M). Bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like modelorganisms for studying of metals influence to metallothionein expression. Metallothionein was isolatedusing fast protein liquid chromatography and quantified by electrochemical methods. The occurrenceof metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15(MT dimer) and 22 kDa (MT trimer).
  • This study was focused on the application of electrochemical methods for studying of bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after theapplication of cadmium and/or lead ions in four concentrations (25, 50, 75 and 150 M). Bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like modelorganisms for studying of metals influence to metallothionein expression. Metallothionein was isolatedusing fast protein liquid chromatography and quantified by electrochemical methods. The occurrenceof metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15(MT dimer) and 22 kDa (MT trimer). (en)
Title
  • An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry
  • An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry (en)
skos:prefLabel
  • An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry
  • An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry (en)
skos:notation
  • RIV/00216305:26620/14:PU111625!RIV15-MSM-26620___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • O, P(ED1.1.00/02.0068)
http://linked.open...iv/cisloPeriodika
  • SI
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 2439
http://linked.open...ai/riv/idVysledku
  • RIV/00216305:26620/14:PU111625
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Escherichia coli, Metallothionein, Voltammetry, Cloning, Metal Ionsa (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [1635596B189D]
http://linked.open...i/riv/nazevZdroje
  • Electrochimica Acta
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 140
http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Hynek, David
  • Kizek, René
  • Křížková, Soňa
  • Novotná, Marie
  • Tmejová, Kateřina
  • Kremplová, Monika
  • Komínková, Markéta
  • Chudobová, Dagmar
  • Pekařík, Vladimír
  • Jimenez Jimenez, Ana Maria
  • Guráň, Roman
  • Číhalová, Kristýna
  • Žůrek, Michal
http://linked.open...ain/vavai/riv/wos
  • 000342528600003
issn
  • 0013-4686
number of pages
http://localhost/t...ganizacniJednotka
  • 26620
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