About: Spectroscopic and Electrochemical Characterization of CD4 Binding Site of HIV-1 Exterior Envelope gp120

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About: Spectroscopic and Electrochemical Characterization of CD4 Binding Site of HIV-1 Exterior Envelope gp120 Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Glycoprotein 120 (gp120) is essential biomolecule for HIV-1 entry into cells as it plays a vital role in attachment to specific cell surface receptors. Exterior envelope glycoprotein 120 contains conservative CD4 binding site in its structure that may be one of target molecules for development of HIV therapeutic agents, able to inhibit the viral entry steps into the host cells. The present study describes the solid-phase, Fmoc-based synthesis of CD4 binding site (SSGGD PEIVMH), and its subsequent spectroscopic characterization, with determined purity over 90 %. Moreover; electrochemical analyses were carried out to optimize the conditions for peptide determination. Using the optimized conditions as Britton-Robinson buffer with pH 8 and 3% addition of acetonitrile (v/v) as a mobile phase, potential 1100 mV, limit of detection of 0.04 ug.mL-1 and limit of quantification of 0.1 ug.mL-1 were estimated Glycoprotein 120 (gp120) is essential biomolecule for HIV-1 entry into cells as it plays a vital role in attachment to specific cell surface receptors. Exterior envelope glycoprotein 120 contains conservative CD4 binding site in its structure that may be one of target molecules for development of HIV therapeutic agents, able to inhibit the viral entry steps into the host cells. The present study describes the solid-phase, Fmoc-based synthesis of CD4 binding site (SSGGD PEIVMH), and its subsequent spectroscopic characterization, with determined purity over 90 %. Moreover; electrochemical analyses were carried out to optimize the conditions for peptide determination. Using the optimized conditions as Britton-Robinson buffer with pH 8 and 3% addition of acetonitrile (v/v) as a mobile phase, potential 1100 mV, limit of detection of 0.04 ug.mL-1 and limit of quantification of 0.1 ug.mL-1 were estimated (en)
Title	Spectroscopic and Electrochemical Characterization of CD4 Binding Site of HIV-1 Exterior Envelope gp120 Spectroscopic and Electrochemical Characterization of CD4 Binding Site of HIV-1 Exterior Envelope gp120 (en)
skos:prefLabel	Spectroscopic and Electrochemical Characterization of CD4 Binding Site of HIV-1 Exterior Envelope gp120 Spectroscopic and Electrochemical Characterization of CD4 Binding Site of HIV-1 Exterior Envelope gp120 (en)
skos:notation	RIV/00216305:26620/14:PU110418!RIV15-MSM-26620___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(EE2.4.31.0023), S
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Heger, Zbyněk Cernei, Natalia Vladimirovna Trnková, Libuše Adam, Vojtěch Kizek, René Zítka, Ondřej Komínková, Markéta Kopel, Pavel Moulick, Amitava Milosavljević, Vedran
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	46578
http://linked.open...ai/riv/idVysledku	RIV/00216305:26620/14:PU110418
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CD4 binding loop, gp120, Human immunodeficiency virus-1, Peptide synthesis (en)
http://linked.open.../riv/klicoveSlovo	Peptide synthesis gp120 CD4 binding loop Human immunodeficiency virus-1
http://linked.open...odStatuVydavatele	RS - Srbská republika
http://linked.open...ontrolniKodProRIV	[71EEAFA1A783]
http://linked.open...i/riv/nazevZdroje	INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	10 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Partner Network for Bionanotechnological and Metallomic Research
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	9
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Cernei, Natalia Vladimirovna Kizek, René Trnková, Libuše Zítka, Ondřej Kopel, Pavel Komínková, Markéta Heger, Zbyněk Moulick, Amitava Milosavljević, Vedran
issn	1452-3981
number of pages	12 (xsd:int)
http://localhost/t...ganizacniJednotka	26620

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