About: Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The technique described in this paper allows detection of quantum dots (QDs) specifically deposited on the polystyrene surface by laser-induced breakdown spectroscopy (LIBS). Using LIBS, the distribution of QDs or their conjugates with biomolecules deposited on the surface can be observed, regardless of the fact if they exhibit fluorescence or not. QDs deposited on the specific surface of polystyrene microplate in the form of spots are detected by determination of the metal included in the QDs structure. Cd-containing QDs (CdS, CdTe) stabilized with mercaptopropionic (MPA) or ercaptosuccinic (MSA) acid, respectively, alone or in the form of conjugates with metallothionein (MT) biomolecule are determined by using the 508.58 nm Cd emission line. The observed absolute detection limit for Cd in CdTe QDs conjugates with MT in one spot was 3 ng Cd. Due to the high sensitivity of this technique, the immunoanalysis in combination with LIBS was also investigated. Cd spatial distribution in sandwich immunoassa The technique described in this paper allows detection of quantum dots (QDs) specifically deposited on the polystyrene surface by laser-induced breakdown spectroscopy (LIBS). Using LIBS, the distribution of QDs or their conjugates with biomolecules deposited on the surface can be observed, regardless of the fact if they exhibit fluorescence or not. QDs deposited on the specific surface of polystyrene microplate in the form of spots are detected by determination of the metal included in the QDs structure. Cd-containing QDs (CdS, CdTe) stabilized with mercaptopropionic (MPA) or ercaptosuccinic (MSA) acid, respectively, alone or in the form of conjugates with metallothionein (MT) biomolecule are determined by using the 508.58 nm Cd emission line. The observed absolute detection limit for Cd in CdTe QDs conjugates with MT in one spot was 3 ng Cd. Due to the high sensitivity of this technique, the immunoanalysis in combination with LIBS was also investigated. Cd spatial distribution in sandwich immunoassa (en)
Title	Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy (en)
skos:prefLabel	Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy (en)
skos:notation	RIV/00216305:26620/14:PU110351!RIV15-MSM-26620___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Křížková, Soňa Novotný, Karel Adam, Vojtěch Kizek, René Kaiser, Jozef Novotná, Marie Kopel, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	20308
http://linked.open...ai/riv/idVysledku	RIV/00216305:26620/14:PU110351
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Laser-induced breakdown spectroscopy, Cadmium, Quantum dots, Metallothionein, Immunoassay (en)
http://linked.open.../riv/klicoveSlovo	Laser-induced breakdown spectroscopy Immunoassay Metallothionein Cadmium Quantum dots
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[2F71E845370E]
http://linked.open...i/riv/nazevZdroje	Spectrochimica Acta Part B
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	101
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Hodek, Petr Kaiser, Jozef Kizek, René Křížková, Soňa Novotný, Karel Novotná, Marie Blažková, Iva Kopel, Pavel
issn	0584-8547
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	26620

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