About: Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads

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About: Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Lactoferrin (LF) is approximately 80 kDa iron-binding protein, which is important part of saliva and other body fluids. Due to its ability to bind metal ions, it has many biologically important functions. In this study, a method for the isolation of LF from a biological sample using robotically prepared antibody-modified paramagnetic particles was developed using robotic pipetting station. The method consisted of the following optimised steps. Protein G was bound on the paramagnetic particles, on which goat antibody (10 microg) was linked. LF was subsequently added to microtitration plate, which had affinity to goat antibody and the interaction lasted for 30 min. We found that the highest signals were obtained using the combination of goat antibody 1:3000, murine antibody 1:5000 and conjugate 1:1500 Lactoferrin (LF) is approximately 80 kDa iron-binding protein, which is important part of saliva and other body fluids. Due to its ability to bind metal ions, it has many biologically important functions. In this study, a method for the isolation of LF from a biological sample using robotically prepared antibody-modified paramagnetic particles was developed using robotic pipetting station. The method consisted of the following optimised steps. Protein G was bound on the paramagnetic particles, on which goat antibody (10 microg) was linked. LF was subsequently added to microtitration plate, which had affinity to goat antibody and the interaction lasted for 30 min. We found that the highest signals were obtained using the combination of goat antibody 1:3000, murine antibody 1:5000 and conjugate 1:1500 (en)
Title	Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads (en)
skos:prefLabel	Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads (en)
skos:notation	RIV/00216305:26620/13:PU106075!RIV14-MSM-26620___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(EE2.4.31.0023)
http://linked.open...iv/cisloPeriodika	14
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Křížková, Soňa Adam, Vojtěch Kizek, René Skaličková, Sylvie Zítka, Ondřej Fialová, Dana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	88179
http://linked.open...ai/riv/idVysledku	RIV/00216305:26620/13:PU106075
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Microfluidic tool, elektrochemical detection, lactoferrin (en)
http://linked.open.../riv/klicoveSlovo	lactoferrin Microfluidic tool elektrochemical detection
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[A5E019271514]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Partner Network for Bionanotechnological and Metallomic Research Central european institute of technology
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	34
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Fialová, Dana Kizek, René Křížková, Soňa Skaličková, Sylvie Zítka, Ondřej
issn	0173-0835
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	26620

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