About: Sequences of pandemic-causing viruses isolated and detected by paramagnetic particles coupled with microfluidic system and electrochemical detector

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Detection of the specific sequences of pandemic-causing viral nucleic acids is area attracting numerous scientists worldwide. In this study, we focused on the isolation and detection of oligonucleotide specific sequences of life-threatening viruses (HIV (GAGCAGTGGGAATA), Influenza (TAATAACCATTGGA) and Ebola (ACCTCACTAGAAAA)) by flow injection analysis with electrochemical detection coupled with paramagnetic particles based isolation. All of used viral genomic sequences contained A6 adenine repetition on the 5 end. This modification enables us to bind them on paramagnetic particle anchoring T25 (MPs-(dT)25). The isolated sequences were subsequently hydrolysed by optimized method using microwaves to obtain single adenines. The obtained samples were detected by microfluidic tool based on stopped flow injection analysis with glassy carbon electrode in the presence of Cu(II)/or CuS nanoparticles to enhance sensitivity and to lower detection limit for adenine. Basic detection parameters as accumulation of C Detection of the specific sequences of pandemic-causing viral nucleic acids is area attracting numerous scientists worldwide. In this study, we focused on the isolation and detection of oligonucleotide specific sequences of life-threatening viruses (HIV (GAGCAGTGGGAATA), Influenza (TAATAACCATTGGA) and Ebola (ACCTCACTAGAAAA)) by flow injection analysis with electrochemical detection coupled with paramagnetic particles based isolation. All of used viral genomic sequences contained A6 adenine repetition on the 5 end. This modification enables us to bind them on paramagnetic particle anchoring T25 (MPs-(dT)25). The isolated sequences were subsequently hydrolysed by optimized method using microwaves to obtain single adenines. The obtained samples were detected by microfluidic tool based on stopped flow injection analysis with glassy carbon electrode in the presence of Cu(II)/or CuS nanoparticles to enhance sensitivity and to lower detection limit for adenine. Basic detection parameters as accumulation of C (en)
Title	Sequences of pandemic-causing viruses isolated and detected by paramagnetic particles coupled with microfluidic system and electrochemical detector Sequences of pandemic-causing viruses isolated and detected by paramagnetic particles coupled with microfluidic system and electrochemical detector (en)
skos:prefLabel	Sequences of pandemic-causing viruses isolated and detected by paramagnetic particles coupled with microfluidic system and electrochemical detector Sequences of pandemic-causing viruses isolated and detected by paramagnetic particles coupled with microfluidic system and electrochemical detector (en)
skos:notation	RIV/00216305:26620/13:PU104844!RIV14-GA0-26620___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(GAP102/11/1068)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Adam, Vojtěch Kizek, René Skaličková, Sylvie Zítka, Ondřej Krejčová, Ludmila Kopel, Pavel Merlos Rodrigo, Miguel Ángel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	104748
http://linked.open...ai/riv/idVysledku	RIV/00216305:26620/13:PU104844
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Paramagnetic particles,viral nucleic sequences, pandemic (en)
http://linked.open.../riv/klicoveSlovo	viral nucleic sequences pandemic Paramagnetic particles
http://linked.open...odStatuVydavatele	RS - Srbská republika
http://linked.open...ontrolniKodProRIV	[646E125CF0D1]
http://linked.open...i/riv/nazevZdroje	INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology Nano-Electro-Bio-Tools for Biochemical and Molecularly-Biological Studies of Eukaryotic Cells (NanoBioTECell)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Kizek, René Krejčová, Ludmila Skaličková, Sylvie Zítka, Ondřej Kopel, Pavel Merlos Rodrigo, Miguel Ángel
issn	1452-3981
number of pages	15 (xsd:int)
http://localhost/t...ganizacniJednotka	26620

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