About: Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene

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About: Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Since its first official detection in the Guangdong province of China in 1996, the highly pathogenic avian influenza virus of H5N1 subtype (HPAI H5N1) has reportedly been the cause of outbreaks in birds in more than 60 countries, 24 of which were European. The main issue is still to develop effective antiviral drugs. In this case, single point mutation in the neuraminidase gene, which causes resistance to antiviral drug and is, therefore, subjected to many studies including ours, was observed. In this study, we developed magnetic electrochemical bar code array for detection of single point mutations (mismatches in up to four nucleotides) in H5N1 neuraminidase gene. Paramagnetic particles Dynabeads with covalently bound oligo (dT)25 were used as a tool for isolation of complementary H5N1 chains (H5N1 Zhejin, China and Aichi). For detection of H5N1 chains, oligonucleotide chains of lengths of 12 (+5 adenine) or 28 (+5 adenine) bp labeled with quantum dots (CdS, ZnS and/or PbS) were used. Individual prob Since its first official detection in the Guangdong province of China in 1996, the highly pathogenic avian influenza virus of H5N1 subtype (HPAI H5N1) has reportedly been the cause of outbreaks in birds in more than 60 countries, 24 of which were European. The main issue is still to develop effective antiviral drugs. In this case, single point mutation in the neuraminidase gene, which causes resistance to antiviral drug and is, therefore, subjected to many studies including ours, was observed. In this study, we developed magnetic electrochemical bar code array for detection of single point mutations (mismatches in up to four nucleotides) in H5N1 neuraminidase gene. Paramagnetic particles Dynabeads with covalently bound oligo (dT)25 were used as a tool for isolation of complementary H5N1 chains (H5N1 Zhejin, China and Aichi). For detection of H5N1 chains, oligonucleotide chains of lengths of 12 (+5 adenine) or 28 (+5 adenine) bp labeled with quantum dots (CdS, ZnS and/or PbS) were used. Individual prob (en)
Title	Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene (en)
skos:prefLabel	Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene (en)
skos:notation	RIV/00216305:26620/13:PU104593!RIV14-GA0-26620___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.1.00/02.0068), P(EE2.4.31.0023), P(GAP102/11/1068)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Babula, Petr Trnková, Libuše Hynek, David Adam, Vojtěch Hubálek, Jaromír Kizek, René Krejčová, Ludmila Kopel, Pavel Merlos Rodrigo, Miguel Ángel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	69077
http://linked.open...ai/riv/idVysledku	RIV/00216305:26620/13:PU104593
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	pathogenic avian influenza virus, antiviral drugs, neuraminidase gene, oligonucleotide, nucleic acids (en)
http://linked.open.../riv/klicoveSlovo	neuraminidase gene pathogenic avian influenza virus oligonucleotide antiviral drugs nucleic acids
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[78D059D50705]
http://linked.open...i/riv/nazevZdroje	VIRUSES-BASEL
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	9 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Partner Network for Bionanotechnological and Metallomic Research Central european institute of technology Nano-Electro-Bio-Tools for Biochemical and Molecularly-Biological Studies of Eukaryotic Cells (NanoBioTECell)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	5
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Babula, Petr Hubálek, Jaromír Hynek, David Kizek, René Krejčová, Ludmila Trnková, Libuše Kopel, Pavel Merlos Rodrigo, Miguel Ángel
issn	1999-4915
number of pages	21 (xsd:int)
http://localhost/t...ganizacniJednotka	26620

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