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  • In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine–adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 degrees Celsius, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 ug mL 1. The detection limit (3 signal/noise) was estimated as 15 ng ml 1 of ODN-SH-Cd.
  • In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine–adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 degrees Celsius, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 ug mL 1. The detection limit (3 signal/noise) was estimated as 15 ng ml 1 of ODN-SH-Cd. (en)
Title
  • Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs
  • Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs (en)
skos:prefLabel
  • Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs
  • Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs (en)
skos:notation
  • RIV/00216305:26620/13:PU104592!RIV14-GA0-26620___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED1.1.00/02.0068), P(GA102/08/1546)
http://linked.open...iv/cisloPeriodika
  • 7-8
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 95310
http://linked.open...ai/riv/idVysledku
  • RIV/00216305:26620/13:PU104592
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • paramagnetic particles, DNA, differential pulse voltammetry, square wave voltammetry (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [A69FE4C18F5B]
http://linked.open...i/riv/nazevZdroje
  • CHROMATOGRAPHIA
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http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 76
http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Hubálek, Jaromír
  • Hynek, David
  • Kizek, René
  • Krejčová, Ludmila
  • Trnková, Libuše
  • Kopel, Pavel
issn
  • 0009-5893
number of pages
http://localhost/t...ganizacniJednotka
  • 26620
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