About: Phytochelatin synthase activity as a marker of metal pollution

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The synthesis of phytochelatins is catalyzed by gamma-Glu-Cys dipeptidyl transpeptidase called phytochelatin synthase (PCS). Aim of this study was to suggest a new tool for determination of phytochelatin synthase activity in the tobacco BY-2 cells treated with different concentrations of the Cd(II). After the optimization steps, an experiment on BY-2 cells exposed to different concentrations of Cd(NO3)2 for 3 days was performed. At the end of the experiment, cells were harvested and homogenized. Reduced glutathione and cadmium (II) ions were added to the cell suspension supernatant. These mixtures were incubated at 35 C for 30 min and analysed using high performance liquid chromatography coupled with electrochemical detector (HPLC-ED). The results revealed that PCS activity rises markedly with increasing concentration of cadmium (II) ions. The lowest concentration of the toxic metal ions caused almost three fold increase in PCS activity as compared to control samples. The activity of PCS (270 fkat) in The synthesis of phytochelatins is catalyzed by gamma-Glu-Cys dipeptidyl transpeptidase called phytochelatin synthase (PCS). Aim of this study was to suggest a new tool for determination of phytochelatin synthase activity in the tobacco BY-2 cells treated with different concentrations of the Cd(II). After the optimization steps, an experiment on BY-2 cells exposed to different concentrations of Cd(NO3)2 for 3 days was performed. At the end of the experiment, cells were harvested and homogenized. Reduced glutathione and cadmium (II) ions were added to the cell suspension supernatant. These mixtures were incubated at 35 C for 30 min and analysed using high performance liquid chromatography coupled with electrochemical detector (HPLC-ED). The results revealed that PCS activity rises markedly with increasing concentration of cadmium (II) ions. The lowest concentration of the toxic metal ions caused almost three fold increase in PCS activity as compared to control samples. The activity of PCS (270 fkat) in (en)
Title	Phytochelatin synthase activity as a marker of metal pollution Phytochelatin synthase activity as a marker of metal pollution (en)
skos:prefLabel	Phytochelatin synthase activity as a marker of metal pollution Phytochelatin synthase activity as a marker of metal pollution (en)
skos:notation	RIV/00216305:26620/11:PU95050!RIV12-MSM-26620___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(GA522/07/0692), P(GD204/09/H002), Z(MSM6215712402)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Adam, Vojtěch Kizek, René Zítka, Ondřej
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	220367
http://linked.open...ai/riv/idVysledku	RIV/00216305:26620/11:PU95050
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Phytochelatin synthase activity, Cadmium, Glutathione, High performance liquid chromatography with electrochemical detection, Tobacco BY-2 cell suspension (en)
http://linked.open.../riv/klicoveSlovo	Cadmium Glutathione High performance liquid chromatography with electrochemical detection Phytochelatin synthase activity Tobacco BY-2 cell suspension
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[7AF68917B39E]
http://linked.open...i/riv/nazevZdroje	Journal of Hazardous Materials
http://linked.open...in/vavai/riv/obor	JB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Plant Developmental Biology and Genetics Central european institute of technology Study of thiol compounds production at plants - use in phytoremediation technologies
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	2011 (192)
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Beklová, Miroslava Kizek, René Sobrová, Pavlína Zehnálek, Josef Zítka, Ondřej
http://linked.open...n/vavai/riv/zamer	Veterinary aspects of food safety and quality
issn	0304-3894
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	26620

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