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  • Ergot alkaloids are mycotoxins produced predominantly by fungi of the Claviceps genus, which mainly affect cereals and wild grasses. Ingestion of ergot alkaloids can cause severe health problems in humans and animals. Thus, there is a need for methods that allow determination of these dangerous toxins. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for the simultaneous determination of eight ergot alkaloids: ergosine, ergocornine, ergocryptine and ergocristine and their corresponding epimers as well. Mean recovery, precision, matrix effects and limits of quantification (LOQ) have been assessed for cereal and grass matrices within the method validation. Sample preparation has been based on modified QuEChERS approach. The presented method was used to inspect various feed samples. Ergot alkaloids were found in 31 out of 113 samples investigated. Ergocornine was the most frequently occuring alkaloid, while the highest levels were observed for ergocryptine, ergocris
  • Ergot alkaloids are mycotoxins produced predominantly by fungi of the Claviceps genus, which mainly affect cereals and wild grasses. Ingestion of ergot alkaloids can cause severe health problems in humans and animals. Thus, there is a need for methods that allow determination of these dangerous toxins. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for the simultaneous determination of eight ergot alkaloids: ergosine, ergocornine, ergocryptine and ergocristine and their corresponding epimers as well. Mean recovery, precision, matrix effects and limits of quantification (LOQ) have been assessed for cereal and grass matrices within the method validation. Sample preparation has been based on modified QuEChERS approach. The presented method was used to inspect various feed samples. Ergot alkaloids were found in 31 out of 113 samples investigated. Ergocornine was the most frequently occuring alkaloid, while the highest levels were observed for ergocryptine, ergocris (en)
Title
  • UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed
  • UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed (en)
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  • UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed
  • UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed (en)
skos:notation
  • RIV/00216305:26310/14:PU112268!RIV15-MSM-26310___
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  • 52086
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  • RIV/00216305:26310/14:PU112268
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  • alkaloids, ergot alkaloids, feed (en)
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  • [5478FEC9D8A7]
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  • Brno
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  • Brno
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  • EMEC15 Book of Abstracts
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  • Čáslavský, Josef
  • Čumová, Martina
  • Kosubová, Petra
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http://linked.open.../riv/zahajeniAkce
number of pages
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  • Vysoké učení technické v Brně
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  • 978-80-214-5073-8
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  • 26310
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