About: Optimisation of DGGE nested PCR for red yeasts characterisation

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Carotenogenic yeasts are a diverse group of unrelated organisms (mostly Basidiomycota) that can be found in soil, fresh and marine water, on plants, commonly associated with animals and man-made habitant. In this work the PCR - denaturing gradient gel electrophoresis (DGGE) method was optimized for characterisation of red yeast strains. For this usage, inter-specific variables of strongly conserved sequences of genomic DNA, especially ITS1 and 5.8-ITS2 rDNA regions were amplified. At all, nine of carotenogenic yeast strains of the genus Rhodotorula, Sporobolomyces, Cystofilobasidium and Phaffia were analyzed. Carotenogenic yeasts are a diverse group of unrelated organisms (mostly Basidiomycota) that can be found in soil, fresh and marine water, on plants, commonly associated with animals and man-made habitant. In this work the PCR - denaturing gradient gel electrophoresis (DGGE) method was optimized for characterisation of red yeast strains. For this usage, inter-specific variables of strongly conserved sequences of genomic DNA, especially ITS1 and 5.8-ITS2 rDNA regions were amplified. At all, nine of carotenogenic yeast strains of the genus Rhodotorula, Sporobolomyces, Cystofilobasidium and Phaffia were analyzed. (en)
Title	Optimisation of DGGE nested PCR for red yeasts characterisation Optimisation of DGGE nested PCR for red yeasts characterisation (en)
skos:prefLabel	Optimisation of DGGE nested PCR for red yeasts characterisation Optimisation of DGGE nested PCR for red yeasts characterisation (en)
skos:notation	RIV/00216305:26310/14:PU112150!RIV15-MSM-26310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(LO1211)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Márová, Ivana Kostovová, Iveta Hároniková, Andrea Petrik, Siniša
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta chemická
http://linked.open...dnocenehoVysledku	34919
http://linked.open...ai/riv/idVysledku	RIV/00216305:26310/14:PU112150
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DGGE, PCR, red yeast (en)
http://linked.open.../riv/klicoveSlovo	PCR DGGE red yeast
http://linked.open...ontrolniKodProRIV	[574E17A1FD80]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Materials Research Centre at FCH BUT- Sustainability and Development
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Hároniková, Andrea Márová, Ivana Petrik, Siniša Kostovová, Iveta
http://localhost/t...ganizacniJednotka	26310

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