About: Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Dynamic light scattering (DLS) was used to monitor the coil–globule transition of calf thymus and bacterial DNAs by poly(ethylene glycol) (PEG 600 and PEG 6,000) in aqueous NaCl solutions. The contribution of PEG 600 and PEG 6,000 to DNA coil diameter change was investigated. Compaction of DNA molecules was observed for a PEG 6,000 concentration ranging from 5.5 to 8 %. Hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA), poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) - P(HEMA-co-EDMA), and poly(glycidyl methacrylate) (PGMA) microspheres containing carboxyl groups were used for DNA isolation. The highest DNA yield was achieved using 16 % PEG 6,000 and 2.0 M NaCl. The amount of isolated DNA correlated with the content of carboxyl groups on the microsphere surface. RNA adsorption on the surface of the microspheres tested was also studied. RNA recovery was more than one order lower than DNA recovery under the same conditions. All types of mic Dynamic light scattering (DLS) was used to monitor the coil–globule transition of calf thymus and bacterial DNAs by poly(ethylene glycol) (PEG 600 and PEG 6,000) in aqueous NaCl solutions. The contribution of PEG 600 and PEG 6,000 to DNA coil diameter change was investigated. Compaction of DNA molecules was observed for a PEG 6,000 concentration ranging from 5.5 to 8 %. Hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA), poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) - P(HEMA-co-EDMA), and poly(glycidyl methacrylate) (PGMA) microspheres containing carboxyl groups were used for DNA isolation. The highest DNA yield was achieved using 16 % PEG 6,000 and 2.0 M NaCl. The amount of isolated DNA correlated with the content of carboxyl groups on the microsphere surface. RNA adsorption on the surface of the microspheres tested was also studied. RNA recovery was more than one order lower than DNA recovery under the same conditions. All types of mic (en)
Title	Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres (en)
skos:prefLabel	Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres (en)
skos:notation	RIV/00216305:26310/14:PU108364!RIV15-MSM-26310___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(GP13-32840P), S
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Španová, Alena Trachtová, Štěpánka Rittich, Bohuslav Prettl, Zsolt
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta chemická
http://linked.open...dnocenehoVysledku	45892
http://linked.open...ai/riv/idVysledku	RIV/00216305:26310/14:PU108364
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA compaction, Magnetic microspheres, DNA isolation, RNA, Polymerase chain reaction (PCR) (en)
http://linked.open.../riv/klicoveSlovo	DNA isolation RNA Polymerase chain reaction (PCR) DNA compaction Magnetic microspheres
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[804A6345AA82]
http://linked.open...i/riv/nazevZdroje	FOOD AND BIOPRODUCTS PROCESSING
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	The development of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	92
http://linked.open...iv/tvurceVysledku	Horák, Daniel Rittich, Bohuslav Trachtová, Štěpánka Španová, Alena Prettl, Zsolt Tóth, Judit Gyenis, János
issn	0960-3085
number of pages	26 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.fbp.2014.05.001
http://localhost/t...ganizacniJednotka	26310

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