About: ANALYSIS OF PLASMID DNA ISOLATED FROM STRAINS OF STAPHYLOCOCCUS AUREUS USING ARTIFICIAL NUCLEASES

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Hydrolysis of nucleic acids mediated by artificial nucleases is very important goal in molecular biology, biotechnology and medicine. Some metal and lanthanide ions and their soluble or insoluble complexes (immobilized on solid particles) are attractive as catalysts for DNA cleavage. The aim of this work was to use of neodymium ions for cleavage of plasmid DNAs. Plasmid DNAs originated from strains of Staphylococcus aureus isolated from clinical and food samples were treated by neodymium ions (Nd3+). Plasmid pUC19 was used as model molecule and linearized pUC19/EcoRI was used as the control of the presence of linear form. Cleavage of plasmid DNA was carried out at different temperatures in HEPES (0.05M) buffer. The reaction time was up to 4 hours. The reaction was stopped with 0.5 M EDTA (pH 8.0). Plasmid DNA cleavage efficiency was evaluated by convert the supercoiled circular (ccc) form of plasmid DNA into nicked circular form (oc) and linear (lin) one and was monitored on the gel according to the s Hydrolysis of nucleic acids mediated by artificial nucleases is very important goal in molecular biology, biotechnology and medicine. Some metal and lanthanide ions and their soluble or insoluble complexes (immobilized on solid particles) are attractive as catalysts for DNA cleavage. The aim of this work was to use of neodymium ions for cleavage of plasmid DNAs. Plasmid DNAs originated from strains of Staphylococcus aureus isolated from clinical and food samples were treated by neodymium ions (Nd3+). Plasmid pUC19 was used as model molecule and linearized pUC19/EcoRI was used as the control of the presence of linear form. Cleavage of plasmid DNA was carried out at different temperatures in HEPES (0.05M) buffer. The reaction time was up to 4 hours. The reaction was stopped with 0.5 M EDTA (pH 8.0). Plasmid DNA cleavage efficiency was evaluated by convert the supercoiled circular (ccc) form of plasmid DNA into nicked circular form (oc) and linear (lin) one and was monitored on the gel according to the s (en)
Title	ANALYSIS OF PLASMID DNA ISOLATED FROM STRAINS OF STAPHYLOCOCCUS AUREUS USING ARTIFICIAL NUCLEASES ANALYSIS OF PLASMID DNA ISOLATED FROM STRAINS OF STAPHYLOCOCCUS AUREUS USING ARTIFICIAL NUCLEASES (en)
skos:prefLabel	ANALYSIS OF PLASMID DNA ISOLATED FROM STRAINS OF STAPHYLOCOCCUS AUREUS USING ARTIFICIAL NUCLEASES ANALYSIS OF PLASMID DNA ISOLATED FROM STRAINS OF STAPHYLOCOCCUS AUREUS USING ARTIFICIAL NUCLEASES (en)
skos:notation	RIV/00216305:26310/13:PU104111!RIV14-MSM-26310___
http://linked.open...avai/riv/aktivita	S
http://linked.open...avai/riv/aktivity	S
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Doškař, Jiří Rittich, Bohuslav Zovčáková, Monika
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta chemická
http://linked.open...dnocenehoVysledku	60607
http://linked.open...ai/riv/idVysledku	RIV/00216305:26310/13:PU104111
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Artificial nucleases, Staphylococcus aureus (en)
http://linked.open.../riv/klicoveSlovo	Artificial nucleases Staphylococcus aureus
http://linked.open...ontrolniKodProRIV	[265C0BB4DDF0]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Doškař, Jiří Pantůček, Roman Rittich, Bohuslav Španová, Alena Zovčáková, Monika
http://localhost/t...ganizacniJednotka	26310

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