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  • The aim of this study was to evaluate CTAB extraction procedure, Qiagen DNA easy kit, direct homogenization, carboxyl-functionalised magnetic non-porous HEMA based microspheres and combination of the above mentioned methods for DNA isolation from different plants. The DNA was evaluated regarding concentration and purity, relative intactness, amplification in PCR. All methods provided DNA that could be used in downstream PCR applications. Here we provide an optimized protocol for isolation of DNA from different plant species by combination of direct homogenization and magnetic microspheres coated by carboxyl groups.
  • The aim of this study was to evaluate CTAB extraction procedure, Qiagen DNA easy kit, direct homogenization, carboxyl-functionalised magnetic non-porous HEMA based microspheres and combination of the above mentioned methods for DNA isolation from different plants. The DNA was evaluated regarding concentration and purity, relative intactness, amplification in PCR. All methods provided DNA that could be used in downstream PCR applications. Here we provide an optimized protocol for isolation of DNA from different plant species by combination of direct homogenization and magnetic microspheres coated by carboxyl groups. (en)
Title
  • Evaluations of methods for DNA isolation from plant samples
  • Evaluations of methods for DNA isolation from plant samples (en)
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  • Evaluations of methods for DNA isolation from plant samples
  • Evaluations of methods for DNA isolation from plant samples (en)
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  • RIV/00216305:26310/13:PU104050!RIV14-MSM-26310___
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  • RIV/00216305:26310/13:PU104050
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  • magnetic particles, plants, foods, polymerase chain reaction, identification (en)
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  • [779C18AFC9E4]
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  • Rittich, Bohuslav
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  • 26310
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