About: Alternative procedure for preparation of Lactobacillus cell lysates

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The Lactobacillus species play an important role in the food industry.They are also very important for human health due to their positive influence of the gastrointestinal tract functions. Identification of the Lactobacillus species is based on the DNA amplification methods. Molecular methods are essential for correct identification of Lactobacillus at the genus or species levels. Polymerase chain reaction (PCR) is common amplification method used in biological laboratories. PCR-ready DNA must be available to amplification. Enzymes and detergents are used for the crude cell lysates preparation. Enzymes disrupt the cell wall and cell proteins and detergents destroy the plasmatic membrane of the cells. DNA is relased into medium and it is available for purification procedure. Lysozyme, sodium dodecyl sulphate (SDS) and proteinase K are the standard components in the bacterial crude lysates preparations. They are relativelly expensive and sophisticated to storage. The aim of this work was find out if e The Lactobacillus species play an important role in the food industry.They are also very important for human health due to their positive influence of the gastrointestinal tract functions. Identification of the Lactobacillus species is based on the DNA amplification methods. Molecular methods are essential for correct identification of Lactobacillus at the genus or species levels. Polymerase chain reaction (PCR) is common amplification method used in biological laboratories. PCR-ready DNA must be available to amplification. Enzymes and detergents are used for the crude cell lysates preparation. Enzymes disrupt the cell wall and cell proteins and detergents destroy the plasmatic membrane of the cells. DNA is relased into medium and it is available for purification procedure. Lysozyme, sodium dodecyl sulphate (SDS) and proteinase K are the standard components in the bacterial crude lysates preparations. They are relativelly expensive and sophisticated to storage. The aim of this work was find out if e (en)
Title	Alternative procedure for preparation of Lactobacillus cell lysates Alternative procedure for preparation of Lactobacillus cell lysates (en)
skos:prefLabel	Alternative procedure for preparation of Lactobacillus cell lysates Alternative procedure for preparation of Lactobacillus cell lysates (en)
skos:notation	RIV/00216305:26310/11:PU94304!RIV12-MSM-26310___
http://linked.open...avai/riv/aktivita	S
http://linked.open...avai/riv/aktivity	S
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Fričová, Michaela Čuta, Robert Španová, Alena Rittich, Bohuslav
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta chemická
http://linked.open...dnocenehoVysledku	185280
http://linked.open...ai/riv/idVysledku	RIV/00216305:26310/11:PU94304
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	crude lysates, Lactobacillus, proteinase K, SDS, DNA (en)
http://linked.open.../riv/klicoveSlovo	DNA Lactobacillus SDS crude lysates proteinase K
http://linked.open...ontrolniKodProRIV	[27FE8F6293FD]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Fričová, Michaela Rittich, Bohuslav Čuta, Robert Španová, Alena
http://localhost/t...ganizacniJednotka	26310

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