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  • Non-selective isolation of high-quality DNA can be performed using carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. Analogically the magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation as well as lanthanum manganese perovskites nanoparticles encapsulated into a thin silica shell.
  • Non-selective isolation of high-quality DNA can be performed using carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. Analogically the magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation as well as lanthanum manganese perovskites nanoparticles encapsulated into a thin silica shell. (en)
Title
  • Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.
  • Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres. (en)
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  • Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.
  • Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres. (en)
skos:notation
  • RIV/00216305:26310/10:PU93095!RIV12-MSM-26310___
http://linked.open...avai/riv/aktivita
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  • P(2B08070)
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  • 285129
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  • RIV/00216305:26310/10:PU93095
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  • magnetic hydrophilic microspheres, P(HEMA-co-GMA), perovskites nanoparticles, PCR-ready DNA, PCR (en)
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  • [62C3F57BEA1A]
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  • Horák, Daniel
  • Rittich, Bohuslav
  • Trachtová, Štěpánka
  • Španová, Alena
  • Tvrdíková, Jana
http://localhost/t...ganizacniJednotka
  • 26310
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